209916-07-4Relevant academic research and scientific papers
Precise control of RNA cleavage by ribozyme mimics
Daniher, Andrew T.,Bashkin, James K.
, p. 1077 - 1078 (1998)
A highly-modified DNA building block, lacking both sugar and base moieties, is synthesized and incorporated into oligonucleotides to form functional mimics of ribozymes.
Metal-assisted hybridization of oligonucleotides, evaluation of circular 2′-O-Me RNA as ligands for the TAR RNA target
Zapata, Laurence,Bathany, Katell,Schmitter, Jean-Marie,Moreau, Serge
, p. 1022 - 1028 (2003)
Two complementary oligonucleotides were conjugated with terpyridine ligands at their nearby 5′- and 3′-ends. Addition of a stoichiometric amount of a transition metal (Zn2+, Fe2+) resulted in a large increase in the melting temperature of the duplex. The conjugation of TPY to stem-loop oligomers provided an efficient procedure for the cyclisation of the oligomer after the addition of metal ions. Such a short stem-loop oligomer was designed to target the HIV-1 TAR RNA through loop-loop interactions. The addition of Zn2+ ions yielded a good ligand (Kd = 30 nM) for this RNA structural element. ( Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2003).
