214541-16-9Relevant academic research and scientific papers
Nucleosides and nucleotides. 182. Synthesis of branched oligodeoxynucleotides with pentaerythritol at the branch point and their thermal stabilization of triplex formation
Ueno,Takeba,Mikawa,Matsuda
, p. 1211 - 1217 (2007/10/03)
To find an oligodeoxynucleotide (ODN) with triplex stabilization capability, we designed and synthesized novel branched ODNs 1 and 2 with pentaerythritols at their branch points. Branched ODNs 1 and 2 were synthesized on a solid support using bis(phosphoramidite) 11. The stability of the triplexes formed by branched ODNs 1 and 2 with (dA)21 or (dT)21, was studied by thermal denaturation. Branched ODN 1 formed a stable parallel T-AT-type triplex with (dA)21 (T(m) = 38.9 °C) in a buffer of 0.01 M sodium phosphate (pH 7.0) containing 0.5 M NaCl and 0.02 M MgCl2, while branched ODN 2 formed a stable antiparallel A · AT-type triplex with (dT)21 (T(m) = 44.2 °C) in the same buffer. The formation of these triplexes was also confirmed by circular dichroism (CD) measurements and a gel retardation assay.
