2149582-14-7Relevant academic research and scientific papers
Synthesis of Sialic Acids, Their Derivatives, and Analogs by Using a Whole-Cell Catalyst
Lv, Xun,Cao, Hongzhi,Lin, Baixue,Wang, Wei,Zhang, Wande,Duan, Qian,Tao, Yong,Liu, Xue-Wei,Li, Xuebing
, p. 15143 - 15149 (2017/10/17)
Sialic acids (Sias) are important constituents of cell surface glycans. Ready access to Sias in large quantities would facilitate the development of carbohydrate-based vaccines and small-molecule drugs. We now present a facile method for synthesizing various natural forms and non-natural derivatives or analogs of Sias by using a whole-cell catalyst, which is constructed by adding a plasmid containing necessary enzyme genes into a metabolically engineered strain of Escherichia coli. The flexible substrate tolerance of incorporated enzymes (N-acetylglucosamine 2-epimerase and N-acetylneuraminic acid aldolase) allows the cellular catalyst to convert a wide range of simple and inexpensive sugars into various Sia-related compounds through an easily scalable fermentation process. Further, syntheses using this whole-cell biotransformation in combination with three conventional enzymatic reactions provide a series of complex Sia-containing glycans (sialyloligosaccharides) and their derivatives bearing different substituents. The processes described herein should permit the large-scale and economical production of both Sias and sialyloligosaccharides, and may complement existing chemical and enzymatic strategies.
Convenient enzymatic synthesis of a p-nitrophenyl oligosaccharide series of sialyl N-acetyllactosamine, sialyl Lex and relevant compounds
Zeng, Xiaoxiong,Uzawa, Hirotaka
, p. 2469 - 2475 (2007/10/03)
From the β-d-Gal-(1→4)-β-d-GlcNAc-OC6H 4NO2-p (1) prepared by the transglycosylation of β-galactosidase from Bacillus circulans, α-d-Neu5Ac-(2→3)- β-d-Gal-(1→4)-β-d-GlcNAc-OC6H4NO 2-p (9) a
