216692-02-3

Upstream product

• 10416-59-8 N,O-bis-(trimethylsilyl)-acetamide 
• 131501-59-2 Diisopropyl-phosphoramidous acid (2R,3R,4R,5R)-2-[bis-(4-methoxy-phenyl)-phenyl-methoxymethyl]-5-(2,4-dioxo-3,4-dihydro-2H-pyrimidin-1-yl)-4-[1-(2-fluoro-phenyl)-4-methoxy-piperidin-4-yloxy]-tetrahydro-furan-3-yl ester 2-cyano-ethyl ester 

Relevant academic research and scientific papers

A novel solid support for synthesis of 2',3'-cyclic phosphate terminated oligonucleotides

Michaelis-Arbusov chemistry was used to prepare O,S-dialkyl 3'-O- nucleosidyl phosphorothiolate triesters in solution and attached to CPG. The support-bound nucleoside was utilised in the synthesis of a pentaribonucleotide that was fully deprotected on the support. Subsequent treatment with a buffered solution of iodine cleaved the RNA from the CPG with concomitant formation of a terminal 2',3'-cyclic phosphate.