A novel solid support for synthesis of 2',3'-cyclic phosphate terminated oligonucleotides

Article abstract of DOI:10.1016/S0040-4039(98)01734-1

Michaelis-Arbusov chemistry was used to prepare O,S-dialkyl 3'-O- nucleosidyl phosphorothiolate triesters in solution and attached to CPG. The support-bound nucleoside was utilised in the synthesis of a pentaribonucleotide that was fully deprotected on the support. Subsequent treatment with a buffered solution of iodine cleaved the RNA from the CPG with concomitant formation of a terminal 2',3'-cyclic phosphate.