220141-70-8 Usage
Uses
1. Used in Molecular Genetics Research:
5-Fluoroorotic Acid Hydrate 98 is used as a research tool for detecting URA3 gene expression in yeast molecular genetic constructs. It helps identify yeast strains with a mutant URA3 gene, which is crucial for understanding the genetic mechanisms and potential applications in various fields.
2. Used in Selection of Resistant Yeast Strains:
5-Fluoroorotic Acid Hydrate 98 is employed in the selection of resistant strains of Saccharomyces cerevisiae that possess a mutant URA3 gene. This gene mutation allows the yeast to grow in the presence of 5-FOA, making it an essential component in the development of genetically modified yeast strains for various applications.
3. Used in Yeast Media Supplementation:
5-Fluoroorotic Acid Hydrate 98 is used as a supplement in yeast media to study the effects of URA3 gene expression on yeast growth and survival. By supplementing the media with uracil, researchers can observe the growth of yeast strains with a mutant URA3 gene in the presence of 5-FOA, providing valuable insights into the genetic and molecular mechanisms involved.
Check Digit Verification of cas no
The CAS Registry Mumber 220141-70-8 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 2,2,0,1,4 and 1 respectively; the second part has 2 digits, 7 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 220141-70:
(8*2)+(7*2)+(6*0)+(5*1)+(4*4)+(3*1)+(2*7)+(1*0)=68
68 % 10 = 8
So 220141-70-8 is a valid CAS Registry Number.
220141-70-8Relevant articles and documents
Process for producing 5-fluorouracil
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, (2008/06/13)
5-Fluorouraacil is produced by reacting orotic acid with a fluorinating agent and then, decarboxylating the resulting 5-fluoro-orotic acid.
