22882-02-6Relevant articles and documents
Fast Cleavage and Deprotection of Oligonucleotides
Reddy, M. P.,Hanna, N. B.,Farooqui, Firdous
, p. 4311 - 4314 (2007/10/02)
We have developed methylamine/ammonia as a fast cleavage and deprotection reagent which effects complete cleavage of oligonucleotides from the solid support in 5 min at room temperature and complete deprotection in 5 min at 65 deg C.The problem of transamination side products formation, faced with the commonly used dCbz (10percent side product) upon treatment with methylamine/ammonia, has been sucessfully solved by the use of dCac (0.0percent side product).DMT dCac phosphoramidite-methylamine/ammonia system furnished oligonucleotides in equal or superior quality as compared to the other systems.