2304-08-7 Usage
Uses
Used in Enzyme Assays:
Thymidine 5'-4-nitrophenyl phosphate is used as a substrate for various enzyme assays, particularly those involving enzymes that act on thymidine monophosphate (TMP). The 4-nitrophenyl group serves as a colorimetric or fluorometric reporter, allowing for the detection and quantification of enzyme activity.
Used in Biochemical Research:
In the field of biochemical research, thymidine 5'-4-nitrophenyl phosphate is used as a tool to study the mechanisms of enzymes that process TMP, such as kinases and phosphatases. By monitoring the release of the 4-nitrophenyl group, researchers can gain insights into the catalytic properties and substrate specificities of these enzymes.
Used in Drug Development:
Thymidine 5'-4-nitrophenyl phosphate can be employed in the development of new drugs targeting enzymes involved in DNA metabolism. By using thymidine 5'-4-nitrophenyl phosphate as a starting point, chemists can design and synthesize novel inhibitors or activators that modulate the activity of these enzymes, potentially leading to the development of therapeutic agents for various diseases, including cancer and genetic disorders.
Used in Analytical Chemistry:
In analytical chemistry, thymidine 5'-4-nitrophenyl phosphate can be utilized as a reference compound for the development and validation of new methods for the detection and quantification of nucleotides and their derivatives. The presence of the 4-nitrophenyl group makes it an ideal candidate for high-performance liquid chromatography (HPLC), mass spectrometry (MS), and other analytical techniques.
Used in Molecular Biology:
Thymidine 5'-4-nitrophenyl phosphate may also find applications in molecular biology, particularly in the study of DNA replication and repair mechanisms. By incorporating thymidine 5'-4-nitrophenyl phosphate into DNA, researchers can investigate the interactions between DNA polymerases and other proteins involved in these processes, providing valuable information for the understanding of DNA metabolism and the development of new therapeutic strategies.
Check Digit Verification of cas no
The CAS Registry Mumber 2304-08-7 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 2,3,0 and 4 respectively; the second part has 2 digits, 0 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 2304-08:
(6*2)+(5*3)+(4*0)+(3*4)+(2*0)+(1*8)=47
47 % 10 = 7
So 2304-08-7 is a valid CAS Registry Number.
2304-08-7Relevant academic research and scientific papers
Bis(m-nitrophenyl) and bis(p-nitrophenyl) esters and the phosphorodiamidate of thymidine 5'-phosphate as potential sources of intracellular thymidine 5'-phosphate in mouse cells in culture
Chawla,Freed,Hampton
, p. 1733 - 1736 (2007/10/02)
Thymidine 5'-phosphate (TMP) derivatives with masked phosphate groups wer synthesized in tritiated form from [methyl-3H]thymidine. They were of interest as models for 5' nucleotide derivatives that might be able to permeate mammalian cells and then liberate intracellular antimetabolite 5' nucleotides by loss of the masking groups. Mouse L fibroblasts were grown in vitro in the presence of 1 mM 5'-amino-5'-deoxythymidine, which was found to suppress >99% of cellular thymidine kinase activity while inhibiting the rate of cell division by only 30%. The TMP derivatives were less effective than thymidine in labeling the deoxyribonucleic acid (DNA) of the L cells. The labeling was inhibited 95-99% by 5'-amino'5'-deoxythymidine, indicating that it represented incorporation into DNA of [3H]thymidine formed from degradation of the test compounds. No evidence was obtained that the compounds acted as sources of intracellular TMP by cell permeation followed by loss of phosphate blocking groups. Similar studies yielded no evidence that the bis(m-nitrophenyl) ester of TMP produced intracellular TMP by that route in the LM(TK-) strain of L cells that are genetically deficient in thymidine kinase.
