238096-59-8Relevant academic research and scientific papers
Synthesis of multiply labelled ribonucleosides for sequence-specific labelling of oligo-RNA
Milecki, Jan,Foeldesi, Andras,Fischer, Artur,Adamiak, Ryszard W.,Chattopadhyaya, Jyoti
, p. 763 - 783 (2007/10/03)
The synthesis of ribonucleotide blocks multiply labelled with 2H, 13C and 15N for solid support synthesis of sequence specifically labelled RNA is described. Labels were introduced in the ribose ring (13C), C5 position of pyrimidine nucleobases (2H) and exocyclic amino groups (15N) and serve as multiple probes for studying the various physicochemical consequences of physiologically important RNA folding by high-resolution multi-nuclear NMR spectroscopy.
The first example of sequence-specific non-uniformly 13C5 labelled RNA: Synthesis of the 29mer HIV-1 TAR RNA with 13C relaxation window
Milecki, Jan,Zamaratski, Edouard,Maltseva, Tatiana V.,Foeldesi, Andras,Adamiak, Ryszard W.,Chattopadhyaya, Jyoti
, p. 6603 - 6622 (2007/10/03)
A complete synthetic protocol as well as 1H- and 13C-NMR data on the monomer building blocks used for the solid-phase synthesis of specifically 13C-labelled (99 atom % 13C) stem (27A and 43G), bulge (24C) and loop (31U) regions of 29mer HIV-1 TAR RNA hairpin starting from the 13C6-D-glucose are presented. The complex NMR spectra of 13C-labelled monomer building blocks, due to the interaction of various 13C and 1H spins, have been assigned. It has been demonstrated by heteronuclear 2D NMR that the non-uniform labelling of the HIV-1 TAR 29mer RNA achieved herein by chemical synthesis provides an optimal opportunity to perform full T1 and T2 relaxation measurements (the 'NMR Relaxation Window') of each type of sugar-carbons for all four strategically placed 13C-labelled residues in a unique and unprecedented manner because of minimal overlap of 13C resonances compared to uniformly labelled oligo-RNA.
