24703-07-9Relevant academic research and scientific papers
Efficiently regioselective glucosylation of estrogen analogues mediated by fungus Rhizopus oryzae AS 3.2380
Li, Ping,Sun, Chengpeng,Wang, Yan,Wang, Shumei,Yan, Chunyan,Deng, Sa,Huo, Xiaokui,Feng, Lei,Wang, Chao,Tian, Yan,Zhang, Baojing,Ma, Xiaochi
, p. 106 - 110 (2017/04/26)
Compared with estrogens, estrogen 3β-glucosides display more superiority in uptake and metabolism, which are thought to be potential replacement therapy agents for female. In the present research, various estrogen 3β-glucosides (1a–6a) were obtained using the biotransformation experiment of estrogen substrates mediated by Rhizopus oryzae AS 3.2380. The characteristics of estrogens glucosylation with high specificity and regio-selectivity were observed. An optimal incubation medium was determined as the pH 0.25 7.0 (sodium phosphate buffer), 20 0.25 g/L (D-glucose) and 24 0.25 h incubation time with the yields 0.25 > 0.25 70%. A environment friendly preparative method for these glucosides was also established using MCI (Middle chromatogram isolated gel) column chromatography eluted with ethanol aqueous, which could give estrogen 3β-glucosides with the purity 0.25 > 0.25 95%. This study suggested that R. oryzae AS 3.2380 could be an efficient engineering bacteria for the glucosylation of chemical substance. Glucosyltransferase also could be prepared from R. oryzae AS 3.2380.
