26275-69-4Relevant articles and documents
APPLICABILITY OF GLUTAMINYL-tRNA CYCLOTRANSFERASE IN THE PEPTIDE SYNTHESIS
Cerovsky, Vaclav,Jost, Karel
, p. 2310 - 2318 (2007/10/02)
Peptides with amino-terminal glutamine and their derivatives on treatment with crude papain preparation, containing glutaminyl-tRNA cyclotransferase (E.C. 2.3.2.5.), are converted into the corresponding compounds with pyroglutamic acid.This enzymic cyclization does not take place with glutamic acid or its γ-derivatives.The reaction has been optimized for the preparation of pyroglutamic acid phenylhydrazide.Glutaminyl-leucine phenylhydrazide and glutaminyl-histidyl-proline amide reacted to give pyroglutamyl-leucine phenylhydrazide and TRH, respectively.Treatment of crude papain with iodoacetamide completely inhibited its activity without affecting the glutaminyl-cyclotransferase activity.