27025-41-8Relevant articles and documents
Interaction between Glutathione and Resveratrol in the Presence of Hydrogen Peroxide: A Kinetic Model
Zinatullina,Kasaikina,Khrameeva,Indeykina,Kononikhin
, p. 255 - 263 (2021/04/26)
Abstract: The kinetics of interaction between glutathione (GSH) and unsaturated phenol resveratrol (RVT) in deionized water in the presence of hydrogen peroxide (H2O2) is studied. At a physiological concentration (0.1–10 mM), GSH containing two carboxyl groups forms acidic solutions (pH of 3–4); the GSH molecules are associated into dimers. Under these conditions, GSH is quite slowly oxidized by atmospheric oxygen, and the reaction between GSH and H2O2 is accompanied by the formation of radicals. The thiyl radical initiation rate (Wi) is a few fractions of a percent of the GSH consumption rate; however, it is sufficient to initiate a thiol–ene chain reaction between GSH and RVT. Using the experimental data on the kinetics and the product composition and the published data on reactions of GSH with H2O2 and thiyl radicals, a kinetic model of the complex interaction between GSH and RVT in the presence of H2O2 in an aqueous medium at 37°C is proposed. The model includes 19 quasi-elementary reactions with respective rate constants, in particular, the formation of intermediate GSH–H2O2 and GSH–GSH complexes, the formation of radicals, and their subsequent transformations into final products in reactions with RVT and GSH. A computer simulation based on the developed model adequately describes the features of the process kinetics in a wide reactant concentration range.
Growth Factor-Decorated Ti3C2 MXene/MoS2 2D Bio-Heterojunctions with Quad-Channel Photonic Disinfection for Effective Regeneration of Bacteria-Invaded Cutaneous Tissue
Yang, Zhaopu,Fu, Xinliang,Ma, Daichuan,Wang, Yulin,Peng, Liming,Shi, Jiacheng,Sun, Jiyu,Gan, Xueqi,Deng, Yi,Yang, Weizhong
, (2021/11/01)
Phototherapy has recently emerged as a competent alternative for combating bacterial infection without antibiotic-resistance risk. However, owing to the bacterial endogenous antioxidative glutathione (GSH), the exogenous reactive oxygen species (ROS) generated by phototherapy can hardly behave desired antibacterial effect. To address the daunting issue, a quad-channel synergistic antibacterial nano-platform of Ti3C2 MXene/MoS2 (MM) 2D bio-heterojunctions (2D bio-HJs) are devised and fabricated, which possess photothermal, photodynamic, peroxidase-like (POD-like), and glutathione oxidase-like properties. Under near-infrared (NIR) laser exposure, the 2D bio-HJs both yield localized heating and raise extracellular ROS level, leading to bacterial inactivation. Synchronously, Mo4+ ions can easily invade into ruptured bacterial membrane, arouse intracellular ROS, and deplete intracellular GSH. Squeezed between the “ROS hurricane” from both internal and external sides, the bacteria are hugely slaughtered. After being further loaded with fibroblast growth factor-21 (FGF21), the 2D bio-HJs exhibit benign cytocompatibility and boost cell migration in vitro. Notably, the in vivo evaluations employing a mouse-infected wound model demonstrate the excellent photonic disinfection towards bacterial infection and accelerated wound healing. Overall, this work provides a powerful nano-platform for the effective regeneration of bacteria-invaded cutaneous tissue using 2D bio-HJs.
Generation of cyclic glutathione via the thiolactonization of glutathione and identification of a new radical scavenging mechanism
Tsutsui, Ayumi,Morishita, Yuta,Furumachi, Hiyori,Fujimoto, Takashi,Hirai, Rina,Fujita, Tomoyuki,Machinami, Tomoya
, (2021/03/01)
Glutathione (GSH) has two important biological activities, GSH conjugation and radical scavenging. In this work, we determined that GSH can participate in an intramolecular cyclization between the glutamic α-carboxylic acid and the cysteinyl thiol moiety under aqueous conditions. Moreover, we showed that the cyclic glutathione (cGSH) product had more potent radical scavenging activity than GSH. The cGSH radical scavenging activity occurred via a mechanism that differed from that of GSH.