287383-59-9 Usage
Description
Scriptaid is a histone deacetylase inhibitor that has an optimal concentration of 6-8 μM in a cell-based assay, is less toxic than trichostatin A, and works in a wide variety of biological systems. It induces cell cycle arrest in colon cancer cells in culture and inhibits tumor growth in vitro and in vivo. Scriptaid also facilitates the cloning of inbred mouse strains produced by somatic cell nuclear transfer.
Uses
Different sources of media describe the Uses of 287383-59-9 differently. You can refer to the following data:
1. Scriptaid has been used in post activation of oocytes and embryo culture.
2. Histone deacetylase inhibitor. A cell-permeable, relatively non-toxic HDAC inhibitor that facilitates transcriptional activation (TGFβ/Smad4) in both stable and transient receptor assays in a concentration-dependent manner.
General Description
Scriptaid is a histone deacetylase (HDAC) inhibitor, which enhances global acetylation of histones. It improves transcriptional activity and protein expression. Scriptaid inhibits tumor growth. It decreases telomerase activity and stimulates glioma cell apoptosis.
Biological Activity
Novel histone deacetylase inhibitor. Produces > 100-fold increase in histone acetylation and displays relatively low toxicity.
Biochem/physiol Actions
Histone deacetylase inhibitor with lower toxicity than trichostatin A; used to enhance protein expression.
References
1) Su?et al.?(2000),?A novel histone deacetylase inhibitor identified by high-throughput transcriptional screening of a compound library; Cancer Res.,?60?3137
2) Huber?et al.?(2011),?Inhibitors of histone deacetylases: correlation between isoform specificity and reactivation of HIV type 1 (HIV-1) from latently infected cells; J. Biol. Chem.,?286?22211
3) Lee?et al.?(2008),?Histone deacetylase inhibitor scriptaid induces cell cycle arrest and epigenetic change in colon cancer cells; J. Oncol.,?33?767
4) Rissi?et al.?(2019),?Inhibition of RNA synthesis during Scriptaid exposure enhances gene reprogramming in SCNT embryos; Reproduction,?157?123
5) Huang?et al.?(2020),?Scriptaid/exercise-induced lysine acetylation is another type of posttranslational modification occurring in titin; J. Appl. Physiol.,?128?276
Check Digit Verification of cas no
The CAS Registry Mumber 287383-59-9 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 2,8,7,3,8 and 3 respectively; the second part has 2 digits, 5 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 287383-59:
(8*2)+(7*8)+(6*7)+(5*3)+(4*8)+(3*3)+(2*5)+(1*9)=189
189 % 10 = 9
So 287383-59-9 is a valid CAS Registry Number.
InChI:InChI=1/C18H18N2O4/c21-15(19-24)10-2-1-3-11-20-17(22)13-8-4-6-12-7-5-9-14(16(12)13)18(20)23/h4-9,24H,1-3,10-11H2,(H,19,21)
287383-59-9Relevant articles and documents
A fluorescent histone deacetylase (HDAC) inhibitor for cellular imaging
Fleming, Cassandra L.,Ashton, Trent D.,Nowell, Cameron,Devlin, Mark,Natoli, Anthony,Schreuders, Jeannette,Pfeffer, Frederick M.
supporting information, p. 7827 - 7830 (2015/05/13)
Fluorescence microscopy studies using 4-morpholinoscriptaid (4MS) demonstrated rapid cellular uptake of this scriptaid analogue into the cytoplasm but no nuclear penetration. As 4MS and scriptaid have the same in vitro activity against HDACs and KASUMI-1 cells; 4MS exemplifies a rational approach to subtly modify 'profluorogenic' substrates for intracellular studies.
A convenient synthesis of the new histone deacetylase inhibitor scriptaid
Gerova, Mariana S.,Petrov, Ognyan I.
, p. 76 - 79 (2014/03/21)
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