32339-66-5Relevant academic research and scientific papers
Dual-factor Synergistically Activated ESIPT-based Probe: Differential Fluorescence Signals to Simultaneously Detect α-Naphthyl Acetate and Acid α-Naphthyl Acetate Esterase
Wang, Kui,Feng, Beidou,Yang, Yonggang,Chen, Yuehua,Wang, Yuzhu,Wang, Yafu,Yang, Lin,Jiang, Kai,James, Tony D.,Zhang, Hua
, p. 14471 - 14480 (2021/11/10)
α-Naphthyl acetate esterase (α-NAE) and acid α-naphthyl acetate esterase (ANAE), a class of special esterases, are important for lymphocyte typing and immunocompetence-monitoring. As such, the simultaneous detection of α-NAE and ANAE has become a target to effectively improve the accuracy in lymphocyte typing. Therefore, we developed a dual-factor synergistically activated ESIPT-based probe (HBT-NA) to detect α-NAE and ANAE sensitively, rapidly, and simultaneously in a differential manner. HBT-NA exhibits differential fluorescence signal outputs toward small changes of α-NAE and ANAE activities. HBT-NA displays a weak fluorescence signal at 392 nm over a pH range from 6.0 to 7.4. However, when it interacts with α-NAE(0-25 U) at pH = 7.4, the fluorescence intensity at 392 nm enhanced linearly within 60 s (F392u202fnm/F0392u202fnm= 0.042 C α-NAE+ 1.1,R2= 0.99). Furthermore, HBT-NA emits ratiometric fluorescence signals (F505u202fnm/F392u202fnm) for ANAE(0-25 U) at pH = 6.0 within 2.0 min, exhibiting a good linear relationship (F505u202fnm/F392u202fnm= 0.83C ANAE- 1.75,R2= 0.99). The differential fluorescence signals can be used to simultaneously detect the activities of α-NAE and ANAE in solutions and complex living organisms. More importantly, based on the differential fluorescence signals toward α-NAE and ANAE, T lymphocytes and B lymphocytes could be successfully typed and differentiated among nontyped lymphocytes, facilitating the real-time evaluation of their immune functions using flow cytometry. Hence, HBT-NA could be used for the ultrasensitive detection of the enzyme activities of α-NAE and ANAE, the real-time precise typing of lymphocytes, and the monitoring of immunocompetence.
Systematic color tuning of a family of luminescent azole-based organoboron compounds suitable for OLED applications
Kiprof, Paul,Carlson, Jeffrey C.,Anderson, Derrick R.,Nemykin, Victor N.
, p. 15120 - 15132 (2013/10/22)
A new tunable luminescent family of heterocyclic aromatic compounds containing boron has been prepared and characterized through NMR spectroscopy, mass spectrometry, absorption and fluorescence spectroscopy as well as structural study using single crystal X-ray crystallography. Spectroscopic properties of the new compounds were also studied using DFT and TDDFT computational models that showed very good agreement of the theoretical data with experimental results. Through introduction of auxochromic atoms such as sulfur and extension of the π system of the ligands it is possible to cover a large range of the visible spectrum. Activation and enhancement of the luminescence is achieved by condensation reactions that introduce the boron fragment forming stable, sublimable compounds suitable for OLED applications.
