32949-41-0Relevant articles and documents
Bottom-Up Construction of an Adaptive Enzymatic Reaction Network
Helwig, Britta,van Sluijs, Bob,Pogodaev, Aleksandr A.,Postma, Sjoerd G. J.,Huck, Wilhelm T. S.
supporting information, p. 14065 - 14069 (2018/10/09)
The reproduction of emergent behaviors in nature using reaction networks is an important objective in synthetic biology and systems chemistry. Herein, the first experimental realization of an enzymatic reaction network capable of an adaptive response is reported. The design is based on the dual activity of trypsin, which activates chymotrypsin while at the same time generating a fluorescent output from a fluorogenic substrate. Once activated, chymotrypsin counteracts the trypsin output by competing for the fluorogenic substrate and producing a non-fluorescent output. It is demonstrated that this network produces a transient fluorescent output under out-of-equilibrium conditions while the input signal persists. Importantly, in agreement with mathematical simulations, we show that optimization of the pulse-like response is an inherent trade-off between maximum amplitude and lowest residual fluorescence.
Dipeptidyl-α,β-epoxyesters as potent irreversible inhibitors of the cysteine proteases cruzain and rhodesain
Gonzalez, Florenci V.,Izquierdo, Javier,Rodriguez, Santiago,McKerrow, James H.,Hansell, Elizabeth
, p. 6697 - 6700 (2008/09/17)
The dipeptidyl epoxyesters 3 and 4 are potent, irreversible inhibitors of cruzain and rhodesain.
Synthesis of Ethylamide of Cyclic Undecapeptide Corresponding to the 593 - 603 Sequence of Transmembrane Glycoprotein gp41 of Human Immunodeficiency Virus Type Two
Sidorova, M. V.,Kudryavtseva, E. V.,Molokoedov, A. S.,Ovchinnikov, M. V.,Bespalova, Zh. D.
, p. 582 - 589 (2007/10/03)
Ethylamide of cyclic disulfide of the HIV-2 peptide antigen corresponding to the 593 - 603 sequence of the gp41 protein was synthesized by conventional methods of peptide chemistry in solution.The absence of racemization during fragment condensation was s
