3348-03-6

Usage

Uses

Used in Cell Biology:
6-CARBOXYFLUORESCEIN DIACETATE is used as a cell viability indicator for distinguishing between viable and apoptotic cells. The expression is: 6-CARBOXYFLUORESCEIN DIACETATE is used as a cell viability indicator for [distinguishing viable cells from apoptotic cells] because [live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin].
Used in Molecular Biology:
6-Carboxyfluorescein Diacetate is used as a fluorescent probe for detecting changes in intracellular pH. The expression is: 6-CARBOXYFLUORESCEIN DIACETATE is used as a fluorescent probe for [detecting changes in intracellular pH] because [it is a polyanionic compound that can be used to monitor pH variations within cells].
Used in Flow Cytometry:
6-Carboxyfluorescein Diacetate derivatives can be used as staining agents for flow cytometry to visualize cells undergoing apoptosis or necrosis. The expression is: 6-CARBOXYFLUORESCEIN DIACETATE is used as a staining agent for [visualizing cells undergoing apoptosis or necrosis] in the context of [flow cytometry] because [its derivatives can be used to specifically label and identify these cells].

InChI:InChI=1/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)

Upstream product

• 108-24-7 acetic anhydride 
• 108-46-3 recorcinol 
• 552-30-7 trimellitic Anhydride 
• 3301-79-9 6-carboxyfluorescein 
• 76608-15-6 6-carboxyfluorescein 

Downstream Products

• 1351025-38-1 C₂₂H₁₅NO₆ 
• 1426650-86-3 FAM-C₆-TPP 
• 1426650-85-2 FAM-C₃-TPP 
• 150206-15-8 6-carboxyfluorescein diacetate N-hydroxysuccinimide ester 

Relevant academic research and scientific papers

Separating the isomers - Efficient synthesis of the N-hydroxysuccinimide esters of 5 and 6-carboxyfluorescein diacetate and 5 and 6-carboxyrhodamine B

Diacetate protection of 5 and 6-carboxyfluorescein followed by synthesis of the N-hydroxysuccinimide esters allowed ready separation of the two isomers on a multi-gram scale. The 5 and 6-carboxyrhodamine B N-hydroxysuccinimide esters were also readily synthesised and separated.

NEW TECHNOLOGY TO CONJUGATE THE TACCALONOLIDE MICROTUBULE STABILIZERS WITH LINKERS/PAYLOADS

The present disclosure is concerned with taccalonolide analogs and conjugated taccalonolide analogs useful as cellular probes and in the treatment of, for example, hyperproliferative disorders such as cardiovascular diseases and cancer. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Site-specific, reversible and fluorescent immobilization of proteins on CrAsH-modified surfaces for microarray analytics

A novel technique for protein immobilization onto CrAsH-modified surfaces is presented. This approach enables an efficient, reversible and fluorogenic immobilization of proteins. Moreover, expressed proteins can also be directly immobilized from cellular lysates without prior purification. The immobilized proteins are suitable for protein-protein interaction studies and the fluorescence enhancement upon immobilization can be employed for the direct detection of the immobilized protein without the need for secondary detection methods. This journal is

FLUORESCENT SUBSTRATE FOR DETECTION OF ENZYMATIC ACTIVITY OF NITRILE-RELATED ENZYME

The object of the present invention is to provide a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme. The present invention provides a compound represented by formula (I) and a fluorescent substrate for detecting the enzymatic activity of a nitrile-related enzyme, which comprises the compound.