335595-83-0Relevant academic research and scientific papers
Synthesis of isotopically labeled D-[1′-13C]ribonucleoside phosphoramidites
Saito, Yoshio,Nyilas, Agnes,Agrofoglio, Luigi A.
, p. 83 - 90 (2007/10/03)
The preparation of fully protected labeled diisopropylamino-β-cyanoethyl-[1′-13C]ribonucleoside phosphoramidites with regioisomeric purity is described. We demonstrated in this paper that a regioselective 2′-O-silylation, through a 3′,5′-O-di-tert-butylsilanediyl protection, has been applied for the synthesis of [1′-13C]ribonucleoside phosphoramidite units. This method allowed us to obtain only the desired 2′-O-silyl-3′-O-phosphoramidites avoiding the undesired 3′-O-silyl-2′-O-phosphoramidite nucleosides isolated by standard procedures. This is a suitable procedure to RNA precursors with respect to the isotope-containing precursors.
Efficient synthesis of D-[1′-13c]-ribonucleosides for incorporation into oligo-RNA
Saito,Nyilas,Agrofoglio
, p. 937 - 940 (2007/10/03)
Syntheses of the monomer building blocks used for the solid-phase synthesis of specifically 1′-13C-labeled oligoribonucleotides from the D-[1-13C]ribose is presented. Procedure has been used for the selective formation of 2′-O-silylated ribonucleosides. After 5′-O-dimethoxytritylation, the synthesis of D-[1′-13C] ribonucleoside phosphoramidites has been achieved.
