34692-44-9Relevant academic research and scientific papers
Kinetic analysis of the temperature dependence of the template-directed formation of oligoguanylate from the 5′-phosphorimidazolide of guanosine on a poly(C) template with Zn2+
Kawamura,Umehara
, p. 927 - 935 (2001)
A kinetic study of the temperature dependence of the template-directed formation of oligoguanylate (oligo(G)) on polycytidylic acid (poly(C)) from the 5′-phosphorimidazolide of guanosine (ImpG) has been carried out in the presence of Zn2+ at 40
Unique Catalysis and Regioselectivity Observed in the Poly(C)-Directed RNA Dimer Formation from 2-MeImpG: Kinetic Analysis as a Function of Monomer and Polymer Concentration
Kanavarioti, Anastassia,Baird, Eldon E.,Hurley, T. Brian,Carruthers, Julie A.,Gangopadhyay, Sumana
, p. 8323 - 8333 (2007/10/03)
Polycytidylate, poly(C), serves as a scaffold or template to direct and catalyze the synthesis of long oligoguanylates from guanosine 5′-phosphate 2-methylimidazolide, 2-MeImpG. In the absence of poly(C), small amounts of three isomeric dimers, i.e., the 2′-5′-, the 3′-5′-, and the pyrophosphate-linked, are formed slowly. In the presence of poly(C) oligomers that are primarily 3′-5′-linked are formed quickly and in high yield. Product analysis suggests that the oligomers are elongation products of the 3′-5′-linked dimer, abbreviated D. Assuming that D is formed slowly from two molecules of 2-MeImpG (Scheme 1) and elongates relatively fast, the initial rate of dimerization, d[D]/dt in M h-1, was determined using two independent methods. The first method is based on the approximation that at the onset of the reaction the substrate is consumed only via hydrolysis and dimerization, and thus elongation can be neglected. The second, more accurate, method exploits the assertion that every oligomer was once a 3′-5′-linked dimer. Hence the concentration of D was obtained indirectly from the concentration of the oligomer products. These two methods gave comparable results. Experiments were run in aqueous solution in the presence of 1.0 M NaCl, 0.2 M MgCl2 at pH 7.9 ± 0.1 and 23°C. Controls were run in the absence of poly(C) and in the presence of other polynucleotides. The kinetics were determined as a function of both monomer and polymer concentration the latter expressed in C equivalents. The kinetic data obtained in the presence of poly(C) confirmed an earlier conclusion regarding the remarkable effect of poly(C) on the formation of the 3′-5′-linked diguanylate. Initial dimerization rates were quantitatively correlated using a simple template-directed (TD) model that presumes cooperative binding (two association constants) of 2-MeImpG on poly(C) and reaction between adjacent template-bound molecules. The model allows for the estimation of the association constants and the intrinsic rate constant of dimerization, k2*- Insights into the detailed mechanism are also gained from this analysis. The fact that the proposed model can successfully correlate kinetic data that vary by more than 5000-fold between the slowest and the fastest reaction adds confidence and suggests the suitability of this model for describing TD reactions in general. It is anticipated that similar analysis of other known TD reactions may lead to clues that will facilitate the design of more efficient polynucleotide-synthesizing systems.
One-Pot Synthesis of α,γ-Dinucleoside 5'-Triphosphates, G5'pppG and A5'pppA, Using S,S'-Bis(4-chlorophenyl)phosphorodithioate
Fukuoka, Koichiro,Suda, Fuminori,Suzuki, Ryo,Ishikawa, Masahide,Hata, Tsujiaki
, p. 499 - 502 (2007/10/02)
S,S'-Bis(4-chlorophenyl) phosphorodithioate was useful for the synthesis of α,γ-dinucleoside 5'-triphosphates, G5'pppG and A5'pppA starting from the corresponding unprotected nucleoside 5'-phosphates under neutral conditions. G5'pppG was used for the synthesis of m7G5'pppG by means of the N7-methylation of one of two guanine moieties of G5'pppG.
