3495-63-4Relevant academic research and scientific papers
Engineering of Nucleic Acids and Synthetic Cofactors as Holo Sensors for Probing Signaling Molecules in the Cellular Membrane Microenvironment
Feng, Guangfu,Luo, Xingyu,Lu, Xu,Xie, Shiyi,Deng, Lu,Kang, Wenyuan,He, Fang,Zhang, Jiaheng,Lei, Chunyang,Lin, Bin,Huang, Yan,Nie, Zhou,Yao, Shouzhuo
supporting information, p. 6590 - 6594 (2019/05/14)
The comprehensive understanding of the mechanisms underlying the interaction of cells with their membrane microenvironment is of great value for fundamental biological research; however, tracking biomolecules on cell surfaces with high temporal and spatial resolution remains a challenge. Herein, a modular strategy is presented for the construction of cell surface DNA-based sensors by engineering DNA motifs and synthetic cofactors. In this strategy, a stimuli-reactive organic molecule is employed as the cofactor for the DNA motif, and the self-assembly of them forms a FRET-based holo DNA-based sensor. With the use of the DNA-based sensors, the versatility of this modular strategy has been demonstrated in the ratiometric imaging of the cellular extrusion process of endogenous signaling molecules, including sulfur dioxide derivatives and nitric oxide.
Shiga toxin-mediated retrograde delivery of a topoisomerase I inhibitor prodrug
El Alaoui, Abdessamad,Schmidt, Frederic,Amessou, Mohamed,Sarr, Marianne,Decaudin, Didier,Florent, Jean-Claude,Johannes, Ludger
, p. 6469 - 6472 (2008/09/17)
(Figure Presented) A retrograde strategy: An innovative cancer-cell delivery concept exploits the naturally evolved characteristics of the Shiga toxin B-subunit (STxB) for the intracellular activation of a newly synthesized prodrug at the level of the bio
Cyclic anti-aggregatory peptides
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, (2008/06/13)
This invention relates to compounds of the formula: STR1 wherein: A' is absent, Asn, Gln, Ala or Abu; A is absent or a D- or L-amino acid chosen from Arg, HArg, (Me2)Arg, (Et2)Arg, Abu, Ala, Gly, His, Lys, or an α-R' substituted deri
Medium-ring Ketone Synthesis. Synthesis of Eight- to Twelve-membered Cyclic Ketones based on the Intramolecular Cyclization of Large-Membered Lactam Sulfoxides or Sulfones
Ohtsuka, Yasuo,Oishi, Takeshi
, p. 454 - 465 (2007/10/02)
An effective general method for the construction of medium-ring ketones from the corresponding ω-bromocarboxylic acids is described.When the diamide disulfide 10 was treated with NaBH4 and NaH in 2-propanol, reductive cleavage of the sulfur-sulfur bond and concomitant intramolecular coupling of the resulting thiolate anion with the terminal bromide took place and the large-ring lactam sulfide 7, which was the key intermediate for the synthesis of medium-ring ketones, was obtained.Although lithium diisopropyl amide (LDA) treatment of 11 followed by reductive desulfurization provided cycloundecanone (13d) in only 19.5percent yield, intramolecular cyclization of the α-methylated analogues 17 with LDA proceeded smoothly and the keto sulfoxides 18 were obtained in quantitative yields.In the case of the lactam sulfones 21, tert-BuOK was effective as a base for the intramolecular cyclization, affording the keto sulfones 22 in quantitative yields.The keto sulfoxides 18 and sulfones 22 were subjected to reductive desulfurization with Al-Hg to yield medium-ring ketones 19 and 13, respectively, in high yields.Keywords - medium-ring ketone; intramolecular cyclization; large-ring lactam sulfide; bis(2-methylaminophenyl) disulfide; 2-methylaminobenzenethiol; reductive desulfurization; sulfur-stabilized carbanion; active methylene
Medium-ring Ketone Synthesis. Intramolecular Acylation of Sulfur-stabilized Carbanions: A Model Study
Ohtsuka, Yasuo,Oishi, Takeshi
, p. 443 - 453 (2007/10/02)
Intramolecular acylation of the sulfur-stabilized carbanions of the acyclic ester 9 and amide sulfides 11 was carried out as a model study for developing an effective method for the construction of medium-ring ketones by ring closure.Reaction of 9a-c or 11a-g with lithium diisopropylamide (LDA) proceeded smoothly and the expected keto sulfides 10a-c or 12a-g, respectively, were obtained.In the cases where R1 and/or R2 in 11 were normal alkyl groups, the reaction did not take place.However, these difficulties were readily overcome either by introducing a methyl group next to the carbonyl group or by converting the sulfides into the corresponding sulfoxides or sulfones.Acylation in the allyl sulfides 11b, d, f and the allyl sulfone 20b takes place at the α-position to the sulfur atom, yielding β,γ-unsaturated ketones.A reductive removal of the sulfide moiety or its conversion into other functional groups was also examined.
