3520-42-1 Usage
Uses
Different sources of media describe the Uses of 3520-42-1 differently. You can refer to the following data:
1. Sulforhodamine B Sodium Salt is a rhodamine dye widely used in nonpermanent tattoos. Studies show that Sulforhodamine B can be used as a potential new therapeutic agent for the treatment of the acute respiratory distress syndrome. Dyes and metabolites, Environmental Testing
2. Sulforhodamine B sodium salt has been used:for relative total cell mass quantificationto investigate the viscosity of coacervatesas a molecular rotor for microviscosity determination in aerosol droplets
3. Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material.
Definition
ChEBI: An organic sodium salt having 4-[3,6-bis(diethylamino)-2,7-dimethylxanthenium-9-yl]benzene-1,3-disulfonate as the counterion.
Preparation
4-Formylbenzene-1,3-disulfonic acid and 3-(Diethylamino)phenol condensation, the products with sulfuric acid dehydration, and ferric chloride oxidation, and finally into sodium salt.
General Description
Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.
Flammability and Explosibility
Notclassified
Biochem/physiol Actions
Sulforhodamine B sodium salt is useful to measure cell biomass and also for cytotoxicity screening.
Properties and Applications
bright blue light pink. Dark red light brown powder. Soluble in water, in blue light red, with fluorescence, also soluble in alcohol. The strong sulfuric acid dyes is orange, diluted for red, with sodium hydroxide solution is blue light red. Mainly used for wool, silk and polyamide fiber dyeing, also can be used for wool and a variety of fabric dyeing, still can be in wool and silk fabric printing directly, also can used for leather dyeing.
Standard
Light Fastness
Soaping
Persperation Fastness
Oxygen bleaching
Fastness to seawater
Fading
Stain
Fading
Stain
Fading
Stain
ISO
3
3-4
3
4
1
2
3
2
AATCC
2-3
3
3
3
1
2
4
2-3
Standard
Light Fastness
Fading
Stain
ISO
3
AATCC
2-3
Check Digit Verification of cas no
The CAS Registry Mumber 3520-42-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,5,2 and 0 respectively; the second part has 2 digits, 4 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 3520-42:
(6*3)+(5*5)+(4*2)+(3*0)+(2*4)+(1*2)=61
61 % 10 = 1
So 3520-42-1 is a valid CAS Registry Number.
InChI:InChI=1/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
3520-42-1Relevant articles and documents
COMPOUND, RESIST COMPOSITION FOR COLOR FILTER, COLOR FILTER, DYED FIBER, AND DYEING METHOD
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Paragraph 0089-0090; 0091, (2018/08/20)
An object is to provide a compound having high color developability and excellent heat resistance, and a resist composition for a color filter and a color filter that contain the dye composition. Another object is to provide a dyed polyamide fiber product having excellent wet rubbing fastness. The objects are achieved by a compound represented by the following general formula (1):
Activatable probes and methods for in vivo gene detection
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, (2008/06/13)
Probes for detecting a target polynucleotide are provided. One aspect provides a molecular beacon probe set wherein the donor molecular beacon comprises a quantum dot and an acceptor molecular beacon comprises at least one reporter. The probes optionally comprise a protein transduction domain, targeting signal, or a combination thereof. Methods for detecting target polynucleotides using the disclosed probes are also provided.
Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
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, (2008/06/13)
The present invention provides labeled nucleic acid amplification oligonucleotides, which can be linear or hairpin primers or blocking oligonucleotides. The oligonucleotides of the invention are labeled with donor and/or acceptor moieties of molecular energy transfer pairs. The moieties can be fluorophores, such that fluorescent energy emitted by the donor is absorbed by the acceptor. The acceptor may be a fluorophore that fluoresces at a wavelength different from the donor moiety, or it may be a quencher. The oligonucleotides of the invention are configured so that a donor moiety and an acceptor moiety are incorporated into the amplification product. The invention also provides methods and kits for directly detecting amplification products employing the nucleic acid amplification primers. When labeled linear primers are used, treatment with exonuclease or by using specific temperature eliminates the need for separation of unincorporated primers. This "closed-tube" format greatly reduces the possibility of carryover contamination with amplification products, provides for high throughput of samples, and may be totally automated.
