36244-70-9Relevant articles and documents
Widely applicable background depletion step enables transaminase evolution through solid-phase screening
Planchestainer, Matteo,Hegarty, Eimear,Heckmann, Christian M.,Gourlay, Louise J.,Paradisi, Francesca
, p. 5952 - 5958 (2019/06/19)
Directed evolution of transaminases is a widespread technique in the development of highly sought-after biocatalysts for industrial applications. This process, however, is challenged by the limited availability of effective high-throughput protocols to evaluate mutant libraries. Here we report a rapid, reliable, and widely applicable background depletion method for solid-phase screening of transaminase variants, which was successfully applied to a transaminase from Halomonas elongata (HEWT), evolved through rounds of random mutagenesis towards a series of diverse prochiral ketones. This approach enabled the identification of transaminase variants in viable cells with significantly improved activity towards para-substituted acetophenones (up to 60-fold), as well as tetrahydrothiophen-3-one and related substrates. Rationalisation of the mutants was assisted by determination of the high-resolution wild-type HEWT crystal structure presented herein.
Synthesis, time-resolved luminescence, nmr spectroscopy, circular dichroism and circularly polarised luminescence studies of enantiopure macrocyclic lanthanide tetraamide complexes
Dickins, Rachel S.,Howard, Judith A.K.,Maupin, Christine L.,Moloney, Janet M.,Parker, David,Riehl, James P.,Siligardi, Giuliano,Williams, J.A. Gareth
, p. 1095 - 1105 (2007/10/03)
The syntheses and properties of a series of lanthanide complexes (Ln = Eu, Tb, Dy, Yb) of C4 symmetric chiral tetraamide ligands based on 1,4,7,10- tetraazacyclododecane are reported. The configuration of the chiral centre at carbon in the amid