40742-23-2Relevant academic research and scientific papers
Universal chromogenic substrates for lipases and esterases
Grognux, Johann,Wahler, Denis,Nyfeler, Erich,Reymond, Jean-Louis
, p. 2981 - 2989 (2007/10/03)
Chromogenic mono- and diacyl-glycerol analogues were prepared and tested as substrates against lipases and esterases in a microtiter plate setup. Thirty-three enzyme samples were analyzed for activity at 100, 10, and 1 μg mL-1. The reaction rat
Enzyme activity fingerprinting with substrate cocktails
Goddard, Jean-Philippe,Reymond, Jean-Louis
, p. 11116 - 11117 (2007/10/03)
In the postgenomic era, emphasis is shifting from protein identification to protein functional analysis. Enzyme function can be characterized by measuring activity across series of substrates, which generates an activity profile or fingerprint. Activity f
Enzyme fingerprints of activity, and stereo- and enantioselectivity from fluorogenic and chromogenic substrate arrays
Wahler, Denis,Badalassi, Fabrizio,Crotti, Paolo,Reymond, Jean-Louis
, p. 3211 - 3228 (2007/10/03)
A series of stereochemically and structurally diverse fluorogenic and chromogenic substrates for hydrolytic enzymes has been synthesized and used to characterize enzyme activity profiles of esterases, lipases, proteases, peptidases, phosphatases, and epoxide hydrolases. The substrates used are particularly resilient to nonspecific reactions due to their mechanism of activation. The activities recorded with the individual substrates are therefore remarkably reproducible, and enable us to use the overall pattern of activity as a specific fingerprint for the enzyme sample. Fingerprints of activity, and enantio- and stereoselectivity are displayed as arrays of color-scale squares that are easily analyzed visually. Such fingerprints might be useful for quality control, enzyme discovery, and possibly for addressing the issue of functional convergence in enzymes.
Novel assay reagents
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, (2008/06/13)
The present invention relates to novel assay reagents, their composition, methods of preparation, and use in the detection and measurement of various biological systems and/or components, e.g. enzymes, antibodies, antigens, and periodate concentration. This invention in its basic form utilizes a class of compounds having, inter alia., a backbone chain, an indicator group, and vicinal oxidizable groups.
