415916-11-9Relevant articles and documents
Synthesis method of O-formate benzyl sulfonamide
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, (2021/10/30)
The invention discloses a synthesis method of ortho-formate benzyl sulfonamide, and relates to the technical field of battery electrolyte additives. The method comprises the following steps: taking 2 - chloromethyl benzoate as a raw material, and performing nucleophilic substitution reaction. The oxidation chlorination reaction, the ammoniation reaction and the tert-butyl protection are obtained, that is, the ortho-formate benzyl sulfonamide. To the invention, a plurality of reaction kettles are used for gradually synthesizing the ortho-formate benzyl sulfonamide, the direct connection relation between each product is isolated, the influence of byproducts generated by the solvent on different reactions is avoided, and the purity of the product is further improved by adopting a method of distilled water washing, ethyl acetate extraction and anhydrous magnesium sulfate drying. The total yield of the synthesis method reaches above 59.96%, and the purity of the ortho-formate benzyl sulfonamide reaches 99.5% or above.
Development of an immunoassay for the residues of the herbicide bensulfuron-methyl.
Lee, Jae Koo,Ahn, Ki Chang,Park, Oee Sook,Ko, Yong Kwan,Kim, Dae-Whang
, p. 1791 - 1803 (2007/10/03)
To develop a competitive indirect enzyme-linked immunosorbent assay based on polyclonal antibodies for the detection of the sulfonylurea herbicide bensulfuron-methyl, seven structurally related haptens were synthesized. Four of them mimicking the target analyte were conjugated to keyhole limpet hemocyanin by the N-hydroxysuccinimide activated ester method to use as immunogens, and all of them were conjugated to bovine serum albumin to use as plate-coating antigens. Polyclonal antibodies raised in rabbits and the coating antigens were screened and selected for the assay in simple homologous and heterologous ELISA formats. Three sensitive heterologous ELISAs were selected and optimized, showing the average IC(50) values of bensulfuron-methyl as low as 0.17, 0.09, and 0.09 ng/mL, the detection ranges of 0.04-0.60, 0.01-0.60, and 0.04-0.25 ng/mL, and the lowest detection limits of 0.03, 0.002, and 0.03 ng/mL, respectively. The cross-reactivities of other sulfonylurea herbicides and metabolites of bensulfuron-methyl to the antibodies were less than 15% in the two assays. Recoveries from the analyte-fortified water samples in assay I were in the range of 81-125% by simple dilution. The correlation between the ELISA and HPLC was 0.999 (n = 15) with a slope of 1.37 in the analysis of groundwater samples fortified with bensulfuron-methyl. The results obtained strongly indicate that the ELISA can be a highly sensitive and convenient tool for detecting bensulfuron-methyl residues in agricultural and environmental samples.
