41614-16-8

Usage

Uses

Used in Pharmaceutical Industry:
4-CHLORO-3-NITROBENZANILIDE is used as an intermediate in the synthesis of various pharmaceuticals and agrochemicals. Its unique structure allows for the development of new drugs with potential therapeutic effects.
Used in Agrochemical Industry:
In the agrochemical industry, 4-CHLORO-3-NITROBENZANILIDE serves as a key intermediate for the production of various agrochemicals, contributing to the development of effective pest control agents and crop protection products.
Used in Research and Development:
4-CHLORO-3-NITROBENZANILIDE is used as a research compound for studying its potential pharmacological properties, such as anti-inflammatory and analgesic effects. This research can lead to the discovery of new therapeutic agents and a better understanding of the compound's biological activities.
Used in Environmental Studies:
4-CHLORO-3-NITROBENZANILIDE has been implicated in environmental contamination, and its potential adverse effects on aquatic organisms are being studied. This research helps to assess the environmental impact and toxicity of the compound, guiding the development of safer and more sustainable chemical alternatives.

InChI:InChI=1/C13H9ClN2O3/c14-11-7-6-9(8-12(11)16(18)19)13(17)15-10-4-2-1-3-5-10/h1-8H,(H,15,17)

Upstream product

• 62-53-3 aniline 
• 38818-50-7 4-chloro-3-nitro-benzoyl chloride 
• 96-99-1 4-chloro-3-nitrobenzoate 

Downstream Products

• 1264954-58-6 C₂₁H₁₅N₃O₃ 
• 1264968-62-8 C₂₂H₁₇N₃O₃ 
• 1264954-33-7 N-(2-amino-4-phenylaminocarbonylphenyl)indole 
• 1264968-65-1 N-(2-amino-4-phenylaminocarbonylphenyl)-3-methylindole 
• 1264954-01-9 C₂₁H₁₄N₄O 
• 1264954-04-2 C₂₂H₁₆N₄O 
• 97-32-5 3-nitro-4-methoxybenzanilide 
• 328258-47-5 4-chloro-N-methyl-3-nitro-N-phenylbenzamide 
• 1268527-54-3 3-ethyl-2-[(E)-2-(methylphenylamino)ethenyl]-1-phenyl-5-phenylcarbamoyl-1H-benzoimidazol-3-ium iodide 
• 1268527-80-5 2-[(E)-2-(methylphenylamino)ethenyl]-1-phenyl-1H-benzoimidazole-5-carboxylic acid phenylamide 
• 1268527-73-6 3-amino-N-phenyl-4-phenylaminobenzamide 

Relevant academic research and scientific papers

Preparation method of 3-amino-4-methoxybenzaniline

The invention relates to a preparation method of 3-amino-4-methoxybenzaniline.The preparation method includes the steps that 3-nitro-4-X-benzoic acid reacts with aniline under a reaction agent condition to obtain 3-nitro-4-X-benzanilide; 2, 3-nitro-4-X-benzanilide reacts with methyl alcohol under the presence of alkaline reagent to obtain 3-nitro-4 methoxybenzaniline; reduction reaction is performed with treatment or without treatment, so that 3-amino-4-methoxybenzaniline is obtained.The method is high in product yield, low in cost, good in quality and convenient to industrialize and meets the requirement for environmentally-friendly production.

Design and synthesis of highly sensitive fluorogenic substrates for glutathione S-transferase and application for activity imaging in living cells

Here we report the development of fluorogenic substrates for glutathione S-transferase (GST), a multigene-family enzyme mainly involved in detoxification of endogenous and exogenous compounds, including drug metabolism. GST is often overexpressed in a variety of malignancies and is involved in the development of resistance to various anticancer drugs. Despite the medical significance of this enzyme, no practical fluorogenic substrates for fluorescence imaging of GST activity or for high-throughput screening of GST inhibitors are yet available. So, we set out to develop new fluorogenic substrates for GST. In preliminary studies, we found that 3,4-dinitrobenzanilide (NNBA) is a specific substrate for GST and established the mechanisms of its glutathionylation and denitration. Using these results as a basis for off/on control of fluorescence, we designed and synthesized new fluorogenic substrates, DNAFs, and a cell membrane-permeable variant, DNAT-Me. These fluorogenic substrates provide a dramatic fluorescence increase upon GST-catalyzed glutathionylation and have excellent kinetic parameters for the present purpose. We were able to detect nuclear localization of GSH/GST activity in HuCCT1 cell lines with the use of DNAT-Me. These results indicate that the newly developed fluorogenic substrates should be useful not only for high-throughput GST-inhibitor screening but also for studies on the mechanisms of drug resistance in cancer cells.