473462-06-5Relevant academic research and scientific papers
Application of deoxycholic acid-based copper-phosphite complexes as ligands in the enantioselective conjugate addition of diethylzinc to acyclic enones
Iuliano, Anna,Scafato, Patrizia
, p. 611 - 618 (2007/10/03)
Four phosphites obtained by linking enantiomerically pure binaphthylchlorophosphite to the two different hydroxy groups of deoxycholic acid were synthesized and used as chiral ligands in the enantioselective copper-catalyzed 1,4-addition of diethylzinc to
Synthesis of [3,4-13C2]-enriched bile salts as NMR probes of protein-ligand interactions
Tochtrop, Gregory P.,DeKoster, Gregory T.,Cistola, David P.,Covey, Douglas F.
, p. 6764 - 6771 (2007/10/03)
Synthetic methodology that allows for incorporation of isotopic carbon at the C-3 and C-4 positions of bile salts is reported. Three [3,4-13C2]-enriched bile salts were synthesized from either deoxycholic or lithocholic acid. The steroid 3α-OH group was oxidized and the A-ring was converted into the Δ4-3-ketone. The C-24 carboxylic acid was next converted into the carbonate group and selectively reduced to the alcohol in the presence of the A-ring enone. Following protection of the 24-OH group, the Δ4-3-ketone was converted into the A-ring enol lactone. Condensation of the enol lactone with [1,2-13C2]-enriched acetyl chloride and subsequent Robinson annulation afforded a [3,4-13C2]-enriched Δ4-3-ketone that was subsequently converted back into a 3α-hydroxy-5β-reduced bile steroid. C-7 hydroxylation, when necessary, was achieved via conversion of the Δ4-3-ketone into the corresponding Δ4,6-dien-3-one, epoxidation of the Δ6-double bond, and hydrogenolysis/hydrogenation of the 5,6-epoxy enone system. The [3,4-13C2]-enriched bile salts were subsequently complexed to human ileal bile acid binding protein (I-BABP), and 1H-13C HSQC spectra were recorded to show the utility of the compounds for investigating the interactions of bile acids with I-BABP.
