5077-24-7Relevant academic research and scientific papers
Formation of 1-Deoxy-D-threo-pentulose and 1-Deoxy-L-threo-pentulose by Cell-free Extracts of Microorganisms
Yokota, Akira,Sasajima, Ken-ichi
, p. 149 - 158 (2007/10/02)
A new enzymatic acyloin-type condensation between pyruvate (or acetoin or methylacetoin) and D-glyceraldehyde was found to be catalyzed by cell-free extracts of a transketolase mutant of Bacillus pumilus IFO 12089.The reaction product (1) was isolated and determined to be 1-deoxy-D-threo-pentulose (D-DTP), which is considered to be a precursor of the five-carbon unit of the thiazole ring thiamine. 1-Deoxy-L-threo-pentulose (L-DTP, 2) was synthesized similarly when L-glyceraldehyde was used instead of D-glyceraldehyde.The configurations of 1 and 2 were confirmed by reduction to the corresponding 1-deoxy-pentitols.Similar enzyme activities were also detected in cell-free extracts of all the wild-type strains tested of bacteria, actinomycetes, yeasts, and molds.These results suggest that the D-DTP synthesizing enzyme plays an important role in the biosynthesis of the thiazole ring of thiamine in vivo.
