511545-98-5

Upstream product

• 50-89-5 thymidine 
• 203510-07-0 1-(2'-deoxy-3'-deutero-β-D-ribofuranosyl)thymine 
• 154371-07-0 5'-O-(4,4'-dimethoxytrityl)-3'-ketothymidine 
• 40615-39-2 5'-O-(4-4'-dimethoxytrityl)thymidine 
• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 124482-92-4 2-cyanoethyl-N,N-(diisopropylamino)chlorophosphine 
• 511546-02-4 1-(2'-deoxy-3'-deutero-5'-O-(4,4'-dimethoxytrityl)-β-D-ribofuranosyl)thymine 

Relevant academic research and scientific papers

A deuterated nucleoside phosphoramidite monomer synthesis method

The invention discloses a synthesis method of a deuterium-substituted nucleoside phosphoramidite monomer. According to the technical scheme, the synthesis method of the deuterium-substituted nucleoside phosphoramidite monomer comprises the following steps

Stereospecific syntheses of 3'-deuterated pyrimidine nucleosides and their site-specific incorporation into DNA.

[reaction: see text] 2'-Deoxy-3'-deutero pyrimidines have been synthesized in high yields and incorporated into deoxyoligonucleotides using standard phosphoramidite chemistry. A key synthetic step is a stereospecific reduction of 3'-keto nucleosides using sodium triacetoxyborodeuteride to give 3'-deuterated thymidine and 2'-deoxy uridine nucleosides. Conversion of the corresponding phorphoramidites 7a and 7b to 4-triazolo derivatives has, for the first time, enabled incorporation of 2'-deoxy-3'-deutero cytidine and 2'-deoxy-3'-deutero-5-methyl cytidine into oligonucleotides.