51549-54-3

Upstream product

• 93966-66-6 N-benzoyl-5'-O-(bis(4-methoxyphenyl)phenylmethyl)-2'-deoxyadenosine 3'-benzoate 
• 958-09-8 2'-deoxy-D-adenosine 
• 98-88-4 benzoyl chloride 
• 93-97-0 benzoic acid anhydride 
• 64325-78-6 N₆-benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyadenosine 

Downstream Products

• 109894-54-4 Benzoic acid (2R,3S,5R)-5-(6-benzoylamino-purin-9-yl)-2-[(2-chloro-phenoxy)-(2-trityloxy-ethylamino)-phosphoryloxymethyl]-tetrahydro-furan-3-yl ester 
• 93966-66-6 N-benzoyl-5'-O-(bis(4-methoxyphenyl)phenylmethyl)-2'-deoxyadenosine 3'-benzoate 
• 84758-11-2 Benzoic acid (2R,3S,5R)-5-(6-benzoylamino-purin-9-yl)-2-[{(2R,3S,5R)-5-(6-benzoylamino-purin-9-yl)-2-[bis-(4-methoxy-phenyl)-phenyl-methoxymethyl]-tetrahydro-furan-3-yloxy}-(2-chloro-phenoxy)-phosphoryloxymethyl]-tetrahydro-furan-3-yl ester 
• 117854-36-1 Benzoic acid (2R,3S,5R)-5-(6-benzoylamino-purin-9-yl)-2-(diisopropylamino-methoxy-phosphanyloxymethyl)-tetrahydro-furan-3-yl ester 
• 114071-89-5 Benzoic acid (2R,3S,5R)-5-(6-benzoylamino-purin-9-yl)-2-{[(2R,3S,5R)-5-(6-benzoylamino-purin-9-yl)-2-(tert-butyl-dimethyl-silanyloxymethyl)-tetrahydro-furan-3-yloxy]-diisopropylamino-phosphoryloxymethyl}-tetrahydro-furan-3-yl ester 
• 114071-87-3 Benzoic acid (2R,3S,5R)-5-(6-benzoylamino-purin-9-yl)-2-[[(2R,3S,5R)-5-(6-benzoylamino-purin-9-yl)-2-(tert-butyl-dimethyl-silanyloxymethyl)-tetrahydro-furan-3-yloxy]-(2,2,2-trifluoro-ethoxy)-phosphoryloxymethyl]-tetrahydro-furan-3-yl ester 
• 91887-21-7 C₆₃H₅₉N₈O₁₆P 
• 93778-56-4 N-benzoyl-P-(4-chlorophenyl)-2'-deoxy-5'-O-(4,4'-dimethoxytrityl)cytidylyl-(3'->5')-N-benzoyl-2'-deoxyadenosine 3'-benzoate 
• 91887-25-1 C₆₉H₆₂N₉O₁₆P 
• 91887-29-5 Benzoic acid (2R,3S,5R)-5-(6-benzoylamino-purin-9-yl)-2-{{(2R,3S,5R)-5-(6-benzoylamino-purin-9-yl)-2-[bis-(4-methoxy-phenyl)-phenyl-methoxymethyl]-tetrahydro-furan-3-yloxy}-[2-(4-nitro-phenyl)-ethoxy]-phosphoryloxymethyl}-tetrahydro-furan-3-yl ester 
• 91887-33-1 C₆₇H₆₄N₁₁O₁₆P 
• 77244-89-4 C₉₆H₇₉Br₆N₁₅O₂₂P₂ 
• 143997-08-4 5'-chloro-5'-deoxy-6-N,3'-O-dibenzoyldeoxyadenosine 

Relevant academic research and scientific papers

Method for removing tetrabutylammonium fluoride

The invention discloses a method for removing tetrabutylammonium fluoride, and belongs to the field of nucleosides. The scheme for removing tetrabutylammonium fluoride can be used for preparing target nucleoside, and the preparation method comprises the following steps: performing selective and positioned hydroxyl protection on a nucleoside initiator by using a silicon protecting group to form a nucleoside modifier; performing a chemical reaction on the nucleoside modifier to prepare a nucleoside precursor carrying the silicon protecting group for protecting the selected hydroxyl group; removing the silicon protecting group in the nucleoside precursor by using tetrabutylammonium fluoride, and hydrolyzing to obtain a to-be-separated substance at least containing the target nucleoside and tetrabutyl quaternary ammonium salt; and separating the tetrabutyl quaternary ammonium salt from the to-be-separated substance by using cation exchange resin to obtain the target nucleoside. According to the scheme, tetrabutylammonium fluoride can be simply and conveniently removed in the preparation process of nucleoside.

COMPOSITIONS AND METHODS FOR CHEMICAL CLEAVAGE AND DEPROTECTION OF SURFACE-BOUND OLIGONUCLEOTIDES

Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

Nucleotide libraries as a source of biologically relevant chemical diversity: Solution-phase synthesis

Solution-phase parallel synthesis of nucleotide library 1 consisting of 150 members is reported. (C) 2000 Elsevier Science Ltd. All rights reserved.

SELF-COMPLEMENTARY TETRADEOXYRIBONUCLEOSIDE TRIPHOSPHATES. CONVENIENT CHEMICAL PREPARATION AND SPECTROSCOPIC STUDIES IN SOLUTION

Eight kinds of self-complementary tetradeoxyribonucleoside triphosphates were prepared by a simplified method which enabled us to omit purification of synthetic intermediates and provided the tetramers very rapidly and conveniently.The tetramers were characterized by enzyme assay and their conformations were studied by the use of UV and CD spectroscopic methods under various conditions.The detailed analysis of the CD spectra suggested that conformation of the tetramer duplexes was dependent on the sequence.

Synthesis of Deoxyoligonucleotide Linker Fragments for Genetic Engineering Using Improved Preparative and Analytical Techniques

For studies on the expression of recombinant DNA, linker fragments have been prepared which serve to introduce specific points of cleavage on the DNA as well as the protein level.Such linkers were designed for cyanogen bromide as well as for collagenase cleavage of fused proteins.The preparation was done according to the triester synthesis scheme using improved and simpler routes to functionalized dinucleotide building blocks.Field desorption mass spectrometry was used as a routine tool for the identification and control of the purity of these units.

Synthesis of 2′-end modified 2′-5′-adenylate trimers

Trimeric 2′,5′-linked adenylates incorporating deoxyribosides and arabinoside of adenine in the 2′-end were synthesized by the phosphotriester approach using quinoline-8-sulfonyl nitrotriazoline as an effective condensing agent.