5181-36-2Relevant articles and documents
15N-D-labeled ionic probes for mass spectrometry
Ito, Fumihiro,Ando, Shin,Iuchi, Masato,Ukari, Taki,Takasaki, Momoka,Yamaguchi, Kentaro
, p. 8009 - 8013 (2011)
An effective 15N- and deuterium (D)-labeled 2,6-bis(oxazolin-2- yl)pyridine (pybox)-La complex based probe ionization method that produces a distinct isotopic shift was developed. The distinct isotopic shift was detected by using the newly synthesized 15N-D-labeled pybox complexes. Moreover, O-[3-(tetramethylpybox)-propyl]-hydroxylamine (oxime-TMpybox) was prepared for attachment to the carbonyl group of the target molecule. Distinct isotopic shifts and multiple charged ions were detected for various compounds having amino, thiol, carboxyl, and carbonyl groups and fullerenes, using the TMpybox ionic probe series in cold-spray ionization mass spectrometry.
A metal-free synthetic approach to peptide-based iminosugar clusters as novel multivalent glycosidase inhibitors
Zelli, Renaud,Bartolami, Eline,Longevial, Jean-Fran?ois,Bessin, Yannick,Dumy, Pascal,Marra, Alberto,Ulrich, Sébastien
, p. 2210 - 2216 (2016)
Multivalent bioconjugates represent emerging tools for enzyme inhibition. In particular, iminosugar clusters have recently shown promising results for the inhibition of glycosidases. However, most of them are prepared by copper-mediated click reactions. We report herein a metal-free approach based on oxime ligation for preparing iminosugar clusters using cyclic and linear tetra-aldehyde peptide scaffolds and oxyamine iminosugars (40-70% yield). Glycosidase inhibition assays revealed the superiority of the clusters made of the linear scaffold, displaying up to a 77-fold increase of relative potency per iminosugar. Thus, this metal-free approach provides a rapid access to structurally-diverse iminosugar clusters for establishing structure-activity relationships in the context of multivalent glycosidase inhibition.
Synthesis method for derivatization reagent containing carbonyl steroid compound (by machine translation)
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Paragraph 0024-0030, (2021/01/04)
The invention relates to a synthesis method for a carbonyl steroid compound derivatization reagent, and belongs to the technical field of chemical synthesis. The synthesis method comprises the following steps: firstly using 1,3 - dibromopropane and N - hydroxyphthalimide to react and synthesize 2 - (3 - bromopropoxy) isoindoline -1, 3 - diketone. 2 - (3 - Bromopropoxy) isoindoline -1, 3 - diketone was reused to prepare 3 - ((1,3 - dioxo -2 - isoindolinyl)-oxy) - N, N, N - trimethyl propyl ammonium bromide. Finally, hydrazinolysis of 3 - ((1,3 - dioxo -2 - isoindolyl)-oxy) - N, N, N - trimethylammonium bromide. The synthesis method is low in cost. The method has the advantages of simple steps, high yield, derivatization of the carbonyl steroid containing compounds, and introduction of the permanent charged groups - N therein. + Me3 , The detection sensitivity of the carbonyl steroid compound in ESI-MS / MS is improved. (by machine translation)
Single-site labeling of histidine in proteins, on-demand reversibility, and traceless metal-free protein purification
Joshi, Pralhad Namdev,Rai, Vishal
supporting information, p. 1100 - 1103 (2019/01/28)
A precision methodology distinguishes one His from all the nucleophilic residues and its multiple copies. An easy-to-operate C-N bond formation labels diverse proteins without adversely affecting their structure and function. The late-stage transformation allows installation of distinct probes. The chemically triggered reversibility enables traceless metal-free purification of proteins with a His-tag.