518977-84-9Relevant academic research and scientific papers
Design and synthesis of a second-generation ligand-tethered calcium indicator for plant cell biology based on the fundamental analyses of the structure and physical property
Takaoka, Yousuke,Imai, Masaki,Shigenaga, Miyuki,Ueda, Minoru
, p. 3079 - 3085 (2017)
Calcium ion (Ca2+) is one of the most important second messengers. However, conventional Ca2+ indicators cannot be applied in plant cells. In previous paper, we developed the first-generation protein-ligand tethered calcium indicator that can be applied for living plant cells. Here, we will report the development of second-generation indicator based on the detailed examination on physical properties of the first-generation to improve the drawbacks. Through the examination, we also found that cell permeability of the probe is strongly affected by their aggregation properties. Our findings will provide new strategy for the design of synthetic indicators.
β-lactams useful in determining the amount of elastase in a clinical sample
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, (2008/06/13)
Disclosed are β-lactams of formula I STR1 wherein X is a chromogenic or fluorogenic substituted aryl or heteroaryl, which are specific inhibitors of Human leukocyte elastase (HLE). Upon contact with HLE these compounds are cleaved to form a chromogenic or fluorogenic species which may be readily measured by the assay disclosed herein. The assay thus provides a means for direct measurement of the amount of active HLE in a body fluid or other sample.
