52659-20-8Relevant academic research and scientific papers
Intracellular Reactions Promoted by Bis(histidine) Miniproteins Stapled Using Palladium(II) Complexes
Gutiérrez-González, Alejandro,Learte-Aymamí, Soraya,Mascare?as, José L.,Vidal, Cristian
, p. 9149 - 9154 (2020)
The generation of catalytically active metalloproteins inside living mammalian cells is a major research challenge at the interface between catalysis and cell biology. Herein we demonstrate that basic domains of bZIP transcription factors, mutated to include two histidine residues at i and i+4 positions, react with palladium(II) sources to generate catalytically active, stapled pallado-miniproteins. The resulting constrained peptides are efficiently internalized into living mammalian cells, where they perform palladium-promoted depropargylation reactions without cellular fixation. Control experiments confirm the requirement of the peptide scaffolding and the palladium staple for attaining the intracellular reactivity.
Preparation method of cresol purple perchlorate
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Paragraph 0018; 0019; 0023; 0024, (2017/08/30)
The invention relates to a preparation method of cresol purple perchlorate and belongs to the field of organic synthesis. The preparation method comprises the following steps: dropwise adding acetic anhydride into an m-aminophenol solution, and maintaining the temperature at 0-5 DEG C for reaction; then adding water and sodium nitrite, dropwise adding phosphoric acid, maintaining the temperature, and carrying out centrifugal separation, and washing, so as to obtain 2-nitroso-5-acetaminophen; and adding an alcohol solvent, dropwise adding perchloric acid, carrying out reflux reaction at a room temperature, cooling, filtering, and drying, so as to obtain cresol purple perchlorate. Compared with a literature synthesis method, the preparation method has the advantages that the reaction steps are simplified, the operation is easy, the product quality is high, and the preparation method has extremely strong process competitive advantages.
