527-42-4Relevant academic research and scientific papers
Identification and characterization of a novel (?)-vibo-quercitol 1-dehydrogenase from Burkholderia terrae suitable for production of (?)-vibo-quercitol from 2-deoxy-scyllo-inosose
Itoh, Nobuya,Kurokawa, Junji,Toda, Hiroshi,Konishi, Kazunobu
, p. 7545 - 7555 (2017/10/10)
(?)-vibo-Quercitol is a deoxyinositol (1l-1,2,4/3,5-cyclohexanepentol) that naturally occurs in oak species, honeydew honey, wines aged in oak barrels, and Gymnema sylvestre and is a potential intermediate for pharmaceuticals. We found that (?)-vibo-quercitol is stereoselectively synthesized from 2-deoxy-scyllo-inosose by the reductive reaction of a novel (?)-vibo-quercitol 1-dehydrogenase found in the proteomes of Burkholderia, Pseudomonas, and Arthrobacter. Among them, Burkholderia terrae sp. AKC-020 (40-1) produced a (?)-vibo-quercitol 1-dehydrogenase appropriate for synthesizing (?)-vibo-quercitol with a high diastereomeric excess. The enzyme was strongly induced in Bu. terrae cells when quercitol or 2-deoxy-scyllo-inosose was used as carbon source in the culture medium. The enzyme is NAD(H)-dependent and shows highly specific activity for (?)-vibo-quercitol and myo-inositol among the substrates tested. The enzyme gene (qudh) was obtained by PCR using degenerate primers based on the N-terminal and internal amino acid sequences of the purified enzyme, followed by thermal asymmetric interlaced PCR. The qudh gene showed homology with inositol 2-dehydrogenase (sharing 49.5% amino acid identity with IdhA from Sinorhizobium meliloti 1021). We successfully produced several recombinant (?)-vibo-quercitol 1-dehydrogenases and related enzymes identified by genome database mining using an Escherichia coli expression system. This revealed that scyllo-inositol dehydrogenase (IolX) in Bacillus subtilis can catalyze the reduction of 2-deoxy-scyllo-inosose to yield scyllo-quercitol, a stereoisomer of (?)-vibo-quercitol. Thus, we successfully identified two enzymes to produce both stereoisomers of deoxyinositols that are rare in nature.
Anti-hepatitis b virus norbisabolane sesquiterpenoids from phyllanthus acidus and the establishment of their absolute configurations using theoretical calculations
Lv, Jun-Jiang,Yu, Shan,Wang, Ya-Feng,Wang, Dong,Zhu, Hong-Tao,Cheng, Rong-Rong,Yang, Chong-Ren,Xu, Min,Zhang, Ying-Jun
, p. 5432 - 5447 (2014/07/08)
Nineteen new highly oxygenated norbisabolane sesquiterpenoids, phyllanthacidoid acid methyl ester (1), and C-T (4-21), were isolated from Phyllanthus acidus Skeels, together with two known ones, phyllanthusols A (2) and B (3), whose sugar moiety was revised as glucosamine-N-acetate, rather than the previously assigned mannosamine-N-acetate. Compounds 2 and 3 were renamed respectively as phyllanthacidoids A (2) and B (3) to avoid confusion. All of the isolates except for 1 are glycosides, whose saccharide moieties possess a pentaoxy cyclohexane (scyllo quercitol) connecting with glucosamine-N-acetate or glucosyl moieties, which are first examples in natural products. Phyllanthacidoids N-R (15-19) with 8R configurations and/or 5,8-diketal skeleton, are unprecedented structures among norbisabolane sesquiterpenoids. Phyllanthacidoids S (20) and T (21) have the unusual tricyclo [3.1.1.1] oxygen bridge skeleton formed by a diketal system, of which the relative configurations of the aliphatic chain were assigned on the basis of heteronuclear coupling constants. The absolute configurations of compounds (1-21) were established by means of calculated electronic circular dichroism (ECD) and coupling constants. Compounds 1-5, 7-9, 10, and 14 displayed potential anti-hepatitis B virus (HBV) activities, with IC50 values of 0.8-36 μM against HBV surface antigen (HBsAg) and HBV excreted antigen (HBeAg), and the results indicated that the 5-ketal group and sugar moieties had contributions to the selectivity of HBsAg and HBeAg.
Stereoselective syntheses of racemic quercitols and bromoquercitols starting from cyclohexa-1,4-diene: Gala-, epi-, muco-, and neo-quercitol
Aydin, G?kay,Savran, Tahir,Akta?, Fatih,Baran, Arif,Balci, Metin
, p. 1511 - 1524 (2013/05/21)
The efficient synthesis of gala-, epi-, neo-, and muco-quercitols and some brominated quercitols starting from cyclohexa-1,4-diene is reported. Treatment of the dibromide, obtained by the addition of bromine to cyclohexa-1,4-diene, with m-chloroperbenzoic
Synthesis of scyllo-inositol derivatives and their effects on amyloid beta peptide aggregation
Sun, Yedi,Zhang, Guohua,Hawkes, Cheryl A.,Shaw, James E.,McLaurin, JoAnne,Nitz, Mark
, p. 7177 - 7184 (2008/12/22)
scyllo-Inositol has shown promise as a potential therapeutic for Alzheimer's disease, by directly interacting with the amyloid β (Aβ) peptide to inhibit Aβ42 fiber formation. To explore the molecular details of the inositol-Aβ42 interaction, a series of scyllo-inositol derivatives have been synthesized which contain deoxy, fluoro, chloro, and methoxy substitutions. The effects of these compounds on the aggregation cascade of Aβ42 have been investigated using electron microscopy (EM). EM analyses revealed that the 1-deoxy-1-fluoro- and 1,4-dimethyl-scyllo-inositols significantly inhibit the formation of Aβ42 fibers. The other derivatives showed some alterations in the morphology of the Aβ42 fibers produced. These findings indicate the importance of all of the hydroxyl groups of scyllo-inositol for complete inhibition of Aβ aggregation.
An efficient and highly stereoselective synthesis of gala-Quercitol from 1,4-cyclohexadiene
Baran, Arif,Secen, Hasan,Balci, Metin
, p. 1500 - 1502 (2007/10/03)
gala-Quercitol was synthesized from 1,4-cyclohexadiene in seven steps and overall yield of 68%. Reaction of 5,6-dibromo-2,2-dimethylhexahydro-1,3-benzodioxole, synthesized from 1,4-cyclohexadiene in three steps, with excess NaOMe gave (3aα,5α,7aα)-5-metho
An Advantageous Synthesis of 1D- and 1L-1,2,3,5/4-Cyclohexanepentol
Biamonte, Marco A.,Vasella, Andrea
, p. 688 - 694 (2007/10/03)
The title compounds D-10 and L-10 were prepared from 1 in eight steps and in a combined overall yield of 41-49%.
A novel synthesis of DL-proto-, and DL-vibo- quercitol via 1,4- cyclohexadiene
Salamci, Emine,Secen, Hasan,Suetbeyaz, Yasar,Balci, Metin
, p. 2223 - 2234 (2007/10/03)
Photooxygenation of 1,4-cyclohexadiene 3 followed by reduction with LiAIH4 or thiourea gave (25/1)-cyclohex-3-ene-triol 7a. trans-Hydroxylation of triol 7a with three different methods afforded both of proto-quercitol 1a and vibo-quercitol 2a.
A concise and convenient synthesis of DL-proto-quercitol and DL-gala-quercitol via ene reaction of singlet oxygen combined with [2 + 4] cycloaddition to cyclohexadiene
Salamci,Secen,Sutbeyaz,Balci
, p. 2453 - 2457 (2007/10/03)
Photooxygenation of 1,4-cyclohexadiene afforded hydroperoxy endoperoxides 3 and 4 in a ratio of 88:12. Reduction of 3 with LiAlH4 or thiourea followed by acetylation of the hydroxyl group and KMnO4 oxidation of the double bond gave proto-quercitol 10b. Application of the same reaction sequences to 4 resulted in the formation of gala-quercitol 14. Quercitols were easily obtained by ammonolysis of acetate derivatives in MeOH. The outcome of dihydroxylation reactions were supported by conformational analysis.
A Stereocontrolled Synthesis of proto-Quercitol
Cambie, Richard C.,Renner, Noel D.,Rutledge, Peter S.,Woodgate, Paul D.
, p. 1597 - 1602 (2007/10/02)
Conduritol A tetramethyl ether (2) has been converted by hydroboration-oxidation into proto-quercitol tetramethyl ether (5) which on demethylation afforded a mixture (2:1) of proto-quercitol (4) and vibo-quercitol (11). proto-Quecitol was also obtained (15percent) from conduritol A tetrabenzoate (3) by a similar sequence.
