58445-96-8Relevant academic research and scientific papers
Secretory phospholipase A2-α from Arabidopsis thaliana: functional parameters and substrate preference
Mansfeld, Johanna,Ulbrich-Hofmann, Renate
, p. 156 - 166 (2008/03/14)
The secretory phospholipase A2-α from Arabidopsis thaliana (AtsPLA2-α), being one of the first plant sPLA2s obtained in purified state, has been characterised with respect to substrate preference and optimum conditions of catalysis. The optima of pH, temperature, and calcium concentration were similar to the parameters of secretory PLA2s from animals. However, substrate preferences markedly differed. In contrast to pancreatic PLA2s, AtsPLA2-α preferred zwitterionic phospholipids, and showed lower activity toward anionic phospholipids. In substrates with two identical fatty acid chains, AtsPLA2-α showed optimum activity toward phospholipids with decanoyl groups. In substrates with palmitoyl groups in sn-1 position, acyl chains with higher degree of unsaturation in sn-2 position were preferred, excluding arachidonic acid, showing the evolutionary adaptation of the enzyme to substrate composition in plants. Km values for short chain phospholipids were comparable to sPLA2s from animals, whereas kcat values were much smaller and interfacial activation was less important.
A practical selective synthesis of mixed short/long chains glycerophosphocholines
D'Arrigo, Paola,Fasoli, Ezio,Pedrocchi-Fantoni, Giuseppe,Rossi, Cristina,Saraceno, Caterina,Tessaro, Davide,Servi, Stefano
, p. 113 - 118 (2008/02/04)
Glycerophosphorylcholine (GPC) is transformed into the cyclic stannylene derivatives, which are selectively acylated to 1-acyl-2-lyso-glycerophosphocholines. The reaction is effective using C-2 to C-16 acid chlorides in 2-propanol. After solvent replacement the lyso-phospholipid (lyso-PL) is subjected to a second acylation using acid anhydrides in methylene chloride. A series of 1(2)-short-2(1)-long-diacyl-glycerophosphocholines are obtained in high yields and selectivity. No diacylation product was detected. In order to detect mixed-chain lipids with inverted disposition of acyl chains, the long chain was introduced first and the thus resulting isomeric compounds compared by NMR. An NMR method was developed in order to determine the positional purity of the isomeric compounds.
