59681-32-2 Usage
Uses
Used in Cell Culture Applications:
L-GLUTAMINE-AMIDE-15N is used as a crucial component of culture media for providing an essential energy source to cells in culture. It is particularly important for optimal cell performance, as it often requires supplementation of the media with L-glutamine prior to use.
Used in Research and Diagnostics:
L-GLUTAMINE-AMIDE-15N is used as a stable isotope-labeled compound in research and diagnostics, allowing for the tracking and analysis of glutamine metabolism and its role in various biological processes.
Used in Pharmaceutical Industry:
L-GLUTAMINE-AMIDE-15N is used as a building block for the synthesis of various pharmaceutical compounds, taking advantage of its unique properties and stable isotope labeling.
Used in Nutritional Supplements:
L-GLUTAMINE-AMIDE-15N is used as an ingredient in nutritional supplements, particularly for athletes and individuals with increased glutamine requirements, to support muscle growth, immune function, and overall health.
Used in Clinical Applications:
L-GLUTAMINE-AMIDE-15N is used in clinical applications to study the role of glutamine in various diseases and conditions, such as gastrointestinal disorders, immune system dysfunction, and muscle wasting. It can also be used to monitor the effectiveness of glutamine supplementation in patients with specific health issues.
Biochem/physiol Actions
L-Glutamine, a source of nitrogen, mainly contributes to the synthesis of proteins, peptides, purine, pyrimidine, nucleotides, nucleic acids and major precursor of neurotransmitter. It acts as a fuel for the proliferation of lymphocytes and macrophages of immune system, for intestinal tissue to maintain gut integrity and for insulin production.(4)(3) L-Glutamine is released by skeletal muscle during stress conditions.
Check Digit Verification of cas no
The CAS Registry Mumber 59681-32-2 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 5,9,6,8 and 1 respectively; the second part has 2 digits, 3 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 59681-32:
(7*5)+(6*9)+(5*6)+(4*8)+(3*1)+(2*3)+(1*2)=162
162 % 10 = 2
So 59681-32-2 is a valid CAS Registry Number.
59681-32-2Relevant academic research and scientific papers
Synthesis of isotope-labeled aminoacids contained in servamycin antibiotic
Ogre',Rimavi,Raap,Shvets
, p. 475 - 479 (2007/10/03)
Approach was developed to a preparative synthesis of isotope-labeled aminoacids contained in servamycin IIB antibiotic. Glutamines labeled with 15N, 13C, and 2H were prepared in 70-80% yield starting with the corresponding labeled glutamic acids under catalysis with the glutamine synthetase enzyme. 15N-2-aminoisohutanoic acid and 15N-isovaline were obtained by Strecker method in 65 and 31% yields respectively. All compounds synthesized were identified and characterized by NMR spectroscopy.
Enzymatic synthesis of specifically 2H-labelled L-glutamic acids and 2H-, 15N-, 13C-labelled L-glutamines on a preparative scale
Ogrel, A.,Vasilenko, I. A.,Lugtenburg, J.,Raap, J.
, p. 369 - 376 (2007/10/02)
In this paper we report the preparation od specifically 2H-labelled L-glutamic acids and 2H-, 15N-, 13C-labelled L-glutamines on the gram scale.The products obtained have high isotope enrichment (>98percent) and high optical purity.The synthetic schemes allow the specific isotope enrichment of every H, C, and N position or any combination of positions. (2-2H)-L-Glutamic acid was synthesized by enantioselective enzymatic conversion of 2-oxoglutaric acid using glutamate dehydrogenase (GDH, E.C. 1.4.1.3.), alcohol dehydrogenase (ADH, E.C. 1.1.1.1.) and (2H6)ethanol. (3,3-2H2)-L-Glutamic acid was prepared by enzymatic conversion of 2-oxo-3,3-dideuteroglutaric acid which was easily obtained from 2-oxoglutaric acid by an isotope exchange reaction in 2H2O at pH 13.0. (4,4-2H2)-L-Glutamic acid was obtained by chemical exchange in 20percent 2HCl.Four different isotopomers of L-glutamine were synthesized by the enantioselective conversion of isotopically labelled L-glutamic acids using glutamine synthetase (GS, E.C. 6.3.1.2.).The amide group of glutamine was labelled with 15N using 15NH4Cl in the enzymatic reaction.The labelled L-glutamic acids and L-glutamines were characterized by 1H-NMR, 13C-NMR and mass spectroscopy.
