628280-42-2

Basic information

• Product Name: N-(9-fluorenylmethyloxycarbonyl)-o-(4,5-dimethoxy-2-nitrobenzyl)-L-serine allyl ester
• Synonyms: N-(9-fluorenylmethyloxycarbonyl)-o-(4,5-dimethoxy-2-nitrobenzyl)-L-serine allyl ester
• CAS NO: 628280-42-2
• Molecular Weight: 562.576
• Mol File: 628280-42-2.mol
• Article Data: 4

Chemical Properties

• CAS DataBase Reference: N-(9-fluorenylmethyloxycarbonyl)-o-(4,5-dimethoxy-2-nitrobenzyl)-L-serine allyl ester(CAS DataBase Reference)
• NIST Chemistry Reference: N-(9-fluorenylmethyloxycarbonyl)-o-(4,5-dimethoxy-2-nitrobenzyl)-L-serine allyl ester(628280-42-2)
• EPA Substance Registry System: N-(9-fluorenylmethyloxycarbonyl)-o-(4,5-dimethoxy-2-nitrobenzyl)-L-serine allyl ester(628280-42-2)

Safety Data

• Hazardous Substances Data: 628280-42-2(Hazardous Substances Data)

Upstream product

• 73724-45-5 N-(9H-fluoren-9-ylmethoxycarbonyl)-L-serine 
• 1016-58-6 6-nitroveratryl alcohol 
• 136497-85-3 N-(9-fluorenylmethoxycarbonyl)-L-serine allyl ester 
• 628280-41-1 4,5-dimethoxy-2-nitrobenzyl trichloroacetimidate 

Downstream Products

• 780009-55-4 (S)-2-Amino-3-(4,5-dimethoxy-2-nitro-benzyloxy)-propionic acid 
• 628280-43-3 N-(9-Fluorenylmethyloxycarbonyl)-O-(4,5-dimethoxy-2-nitrobenzyl)-L-serine 

Relevant articles and documents

An Integrated Chemical Cytometry Method: Shining a Light on Akt Activity in Single Cells

Tools to evaluate oncogenic kinase activity in small clinical samples have the power to guide precision medicine in oncology. Existing platforms have demonstrated impressive insights into the activity of protein kinases, but these technologies are unsuitable for the study of kinase behavior in large numbers of primary human cells. To address these limitations, we developed an integrated analysis system that utilizes a light-programmable, cell-permeable reporter deliverable simultaneously to many cells. The reporter's ability to act as a substrate for Akt, a key oncogenic kinase, was masked by a 2-4,5-dimethoxy 2-nitrobenzyl (DMNB) moiety. Upon exposure to ultraviolet light and release of the masking moiety, the substrate sequence enabled programmable reaction times within the cell cytoplasm. When coupled to automated single-cell capillary electrophoresis, statistically significant numbers of primary human cells were readily evaluated for Akt activity.

Fluorescent assays for protein kinases

This invention provides fluorescently-labeled peptide substrates for protein kinases; methods using the substrates for identifying compounds that inhibit protein kinases, for determining if particular protein kinases are active in cells, for diagnosing di