628280-43-3 Usage
Description
N-Fmoc DMNB-L-serine is a photolytically labile serine derivative that is widely utilized in the field of biochemistry and molecular biology. It is characterized by its ability to be incorporated into short peptides, which can then be used to create caged protein kinase sensors. These sensors are essential for the measurement of intracellular enzymatic activity, providing valuable insights into cellular processes and functions.
Uses
Used in Biochemistry and Molecular Biology:
N-Fmoc DMNB-L-serine is used as a key component in the synthesis of caged protein kinase sensors for monitoring intracellular enzymatic activity. The application reason is its unique photolytic properties, which allow for the controlled release of the serine molecule upon exposure to light, enabling the study of protein kinase activity in living cells.
Used in Pharmaceutical Research:
In the pharmaceutical industry, N-Fmoc DMNB-L-serine is used as a building block for the development of novel drug candidates targeting protein kinases. The application reason is its ability to modulate protein kinase activity, which plays a crucial role in various cellular signaling pathways and is often dysregulated in diseases such as cancer, making it an attractive therapeutic target.
Used in Drug Delivery Systems:
N-Fmoc DMNB-L-serine can also be employed in the design of drug delivery systems, particularly for the targeted delivery of protein kinase inhibitors. The application reason is its compatibility with various drug carrier platforms, such as nanoparticles and liposomes, which can enhance the bioavailability and therapeutic efficacy of the drug candidates.
Check Digit Verification of cas no
The CAS Registry Mumber 628280-43-3 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 6,2,8,2,8 and 0 respectively; the second part has 2 digits, 4 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 628280-43:
(8*6)+(7*2)+(6*8)+(5*2)+(4*8)+(3*0)+(2*4)+(1*3)=163
163 % 10 = 3
So 628280-43-3 is a valid CAS Registry Number.
628280-43-3Relevant articles and documents
An Integrated Chemical Cytometry Method: Shining a Light on Akt Activity in Single Cells
Mainz, Emilie R.,Wang, Qunzhao,Lawrence, David S.,Allbritton, Nancy L.
, p. 13095 - 13098 (2016/10/30)
Tools to evaluate oncogenic kinase activity in small clinical samples have the power to guide precision medicine in oncology. Existing platforms have demonstrated impressive insights into the activity of protein kinases, but these technologies are unsuitable for the study of kinase behavior in large numbers of primary human cells. To address these limitations, we developed an integrated analysis system that utilizes a light-programmable, cell-permeable reporter deliverable simultaneously to many cells. The reporter's ability to act as a substrate for Akt, a key oncogenic kinase, was masked by a 2-4,5-dimethoxy 2-nitrobenzyl (DMNB) moiety. Upon exposure to ultraviolet light and release of the masking moiety, the substrate sequence enabled programmable reaction times within the cell cytoplasm. When coupled to automated single-cell capillary electrophoresis, statistically significant numbers of primary human cells were readily evaluated for Akt activity.
Fluorescent assays for protein kinases
-
, (2010/08/04)
This invention provides fluorescently-labeled peptide substrates for protein kinases; methods using the substrates for identifying compounds that inhibit protein kinases, for determining if particular protein kinases are active in cells, for diagnosing di
