6303-61-3Relevant academic research and scientific papers
A comparative study of the neutral and acidic polysaccharides from Allium macrostemon Bunge
Zhang, Zhanjun,Wang, Fuhua,Wang, Mingchun,Ma, Liping,Ye, Hong,Zeng, Xiaoxiong
, p. 980 - 987 (2015/01/09)
Neutral and acidic polysaccharides, named AMP40N and AMP40S respectively, were isolated and purified from the dried bulbs of Allium macrostemon Bunge. Both of them showed a single and symmetrically sharp peak, indicating they were homogeneous polysaccharides. Molecular weights of AMP40N and AMP40S were determined to be 18.2 and 105.1 kDa, respectively. AMP40N was composed of arabinose and glucose, while AMP40S was composed of rhamnose, arabinose, glucose and galactose and a certain amount of uronic acid. FT-IR, periodic acid oxidation, Smith degradation, methylation and GC-MS analysis revealed that non-reducing terminal and →2,6)-Glc-(1→ existed in AMP40N and AMP40S. The glycosidic linkage of arabinose in AMP40N was →2)-Ara-(1→, whereas it was Ara-(1→ in AMP40S. AMP40S had (1→2)-linked l-rhamnose residue. Both AMP40N and AMP40S exhibited strong anti-tumor potential against human gastric carcinoma cells BGC-823, in particular, AMP40S presented significantly higher inhibitory rate of 85.94% than AMP40N of 52.63%.
A new furostanol saponin from Asparagus cochinchinensis
Shen, Yang,Xu, Cong-Li,Xuan, Wei-Dong,Li, Hui-Liang,Liu, Run-Hui,Xu, Xi-Ke,Chen, Hai-Sheng
experimental part, p. 1587 - 1591 (2012/04/10)
A new furostanol saponin, (25S)-26-O-β-d-glucopyranosyl-5β- furost-20(22)-en-3β, 15β,26-triol-3-O-[α-l-rhamnopyranosyl-(1-4) ]-β-d-glucopyranoside, namely, aspacochioside D (1) were isolated from Asparagus cochinchinensis (Lour.) Merr, along with three known saponins, aspacochioside C (2), (25S)-5β-spirostan-3β-yl-O-[O-α-L- rhamnopyranosyl-(1-4)]-β-d-glucopyranoside (3), and pseudoprotoneodioscin (4). The structure of 1 was elucidated on the basis of chemical reactions and spectral analysis (IR, GC, ESI-MS, 1H-NMR, 13C-NMR, DEPT, HMBC, HMQC and NOESY). The antiproliferative effects of 1-4 were evaluated in a cytotoxicity assay against the human tumor cell line, A549. Compound 2 (Aspacochioside C) exhibited moderate cytotoxicity against A-549, with an IC50 value of 3.87 μg/mL.
New triterpenoid sapoin from Ardisia gigantifolia Stapf.
Gong, Qiang Qiang,Mu, Li Hua,Liu, Ping,Yang, Shi Lin,Wang, Bo,Feng, Yu Lin
experimental part, p. 449 - 452 (2011/02/26)
One new triterpenoid sapoin with two known triterpenoid sapoins: 3β-O-{α-l-rhamnopyranosyl-(1 → 3)-[β-d-xylopyranose-(1 → 2)]-β-d-glucopyranosyl-(1 → 4)-α-l-arabinopyranosyl}-3β-hydroxy-13β,28-epoxy-oleanan-16-oxo-30-al (1), 3β-O-{α-l-rhamnopyranosyl-(1 →
Antioxidant activity of a water-soluble polysaccharide purified from Pteridium aquilinum
Xu, Wentao,Zhang, Fangfang,Luo, YunBo,Ma, Liyan,Kou, Xiaohong,Huang, Kunlun
experimental part, p. 217 - 222 (2009/04/11)
A water-soluble crude polysaccharide, obtained from fern Pteridium aquilinum, was fractionated by DEAE-Sepharose Fast-Flow column chromatography, and purified by Sephacryl S-400 HR column chromatography. The average molecular weight (Mw) of the purified polysaccharide (PLP) is 458,000 Da. The monosaccharide components of PLP were characterized by gas chromatography (GC), and the majority of the monosaccharide components was glucose (relative mass 58.1%) with low levels of galactose, mannose, rhamnose, and arabinose (relative mass 18.7%, 6.8%, 10.2%, and 6.1%, respectively). The Fourier-transform infrared spectra (FTIR) of PLP revealed typical characteristics of polysaccharides. On the basis of the ferric-reducing antioxidant power assay (FRAP), DPPH radical-scavenging, the superoxide radical assay, and self-oxidation of 1,2,3-phentriol assay, the antioxidant activities of PLP were investigated. The purified polysaccharide was demonstrated to have strong reductive power (FRAP value: 827.6 μmol/L), moderate scavenging activities against DPPH radicals (83.1%) and superoxide radicals (60.5%), and moderate inhibiting power for self-oxidation of 1,2,3-phentriol (52.4%).
Acylic sugar derivatives for GC/MS analysis of 13C-enrichment during carbohydrate metabolism
Price, Neil P. J.
, p. 6566 - 6574 (2007/10/03)
Metabolic profiling with stable-isotope tracers in combination with gas chromatography/mass spectrometry (GC/ MS) is a well-established technique for measuring substrate redistribution within metabolic pathways. This analysis relies on the ability to localize and quantify the fractional incorporation of 13C isotope into each carbon atom of precursor-derived metabolites. In this paper, several carbohydrate derivatization procedures (peracetylation, deuterioalditol acetates, and aldononitrile acetates) are evaluated for the positional isotopic information obtained by gas chromatography/electron impact mass spectrometry (GC/EI-MS). These derivatives have been compared for the quantitative evaluation of 13C distribution into isotopomers of 13C-labeled aldoses and ketoses, and the fragmentation pathways for 15 hexoses, pentoses, and amino sugars of biological origin have been assessed. In addition, a new type of carbohydrate derivative (dialkyldithioacetal acetates) has been developed for GC/MS that retains the charge on the anomeric carbon of the original monosaccharide. Electron impact ionization of these derivatives generates well-resolved base peaks arising from C1-C2 bond cleavage with charge retention at the C1 thiol groups. The dialkyldithioacetal acetates are uniquely well suited for measuring isotopic enrichment into the characteristic anomeric carbon of aldose sugars and will facilitate the global analysis of metabolic flux in carbohydrate pathways.
