63224-16-8Relevant articles and documents
Isotope-labeled N, N-dimethyl ethylenediamine, preparation method thereof and analysis method of short-chain fatty acid
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Paragraph 0043-0051; 0054-0055, (2021/06/21)
The invention discloses isotope labeled N, N-dimethyl ethylenediamine, a preparation method thereof and an analysis method of short-chain fatty acid (SCFAs). The isotope labeled N, N-dimethylethylenediamine is 13C2-DMED, and is prepared by the following steps: reacting N-carbobenzoxy ethylenediamine hydrochloride serving as a substrate raw material with H13CHO and NaBH3CN, then carrying out Pd-C catalytic hydrogenation reaction, and finally adding ammonia water, stirring and neutralizing. According to the invention, DMED and 13C2-DMED serve as a pair of isotope labeling reagents, and react with SCFAs to obtain a derivative of the SCFAs; then, the HILIC-MS is used for analyzing and detecting the SCFAs in the biological sample. After the SCFAs is marked by DMED, the detection sensitivity in an ESI positive ion mode is improved by four orders of magnitude. The method is successfully used for high-sensitivity detection of SCFAs in complex matrix samples such as mouse excrement and the like.
INFLUENCE OF THE CONFORMATION OF AMIDE ANALOGS OF ACETYLCHOLINE ON THEIR CHOLINOMIMETIC ACTIVITY
Khromov-Borisov, N. V.,Aleksandrova, L. N.,Danilov, A. F.,Shelkovnikov, S. A.
, p. 401 - 403 (2007/10/02)
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