633-02-3Relevant academic research and scientific papers
Covalent live-cell labeling of proteins using a photoreactive fluorogen
Ayele, Tewoderos M.,Knutson, Steve D.,Heemstra, Jennifer M.
, p. 355 - 377 (2020)
Fluorescence microscopy has dramatically advanced our understanding of the processes that drive biological systems by enabling the imaging and tracking of biomolecules of interest inside of living cells. In particular, proteins of interest can be genetica
Covalent Probes for Aggregated Protein Imaging via Michael Addition
Wan, Wang,Huang, Yanan,Xia, Qiuxuan,Bai, Yulong,Chen, Yuwen,Jin, Wenhan,Wang, Mengdie,Shen, Di,Lyu, Haochen,Tang, Yuqi,Dong, Xuepeng,Gao, Zhenming,Zhao, Qun,Zhang, Lihua,Liu, Yu
, p. 11335 - 11343 (2021/04/19)
Covalent chemical reactions to modify aggregated proteins are rare. Here, we reported covalent Michael addition can generally occur upon protein aggregation. Such reactivity was initially discovered by a bioinspired fluorescent color-switch probe mimickin
Triarylmethane Dyes for Artificial Repellent Cotton Fibers
Montagut, Ana Maria,Gálvez, Erik,Shafir, Alexandr,Sebastián, Rosa María,Vallribera, Adelina
, p. 3810 - 3814 (2017/03/27)
Families of new hydrophobic and/or oleophobic triarylmethane dyes possessing long hydrocarbon or polyfluorinated chains have been prepared. When covalently grafted on to cotton fabric, these dyes give rise to a new type of colored superhydrophobic fibers.
MAGNETIC LABELING OF BACTERIA
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, (2014/01/08)
The present invention provides novel methods of magnetically labeling a bacterial cell by contacting the call with an affinity ligand and subsequently contacting the cell with a magnetic agent, where the affinity ligand and magnetic agent include bioorthogonally reactive groups that can react with each other to form a covalent bond. Compounds, compositions, kits and applications of the method are also described.
A magnetic gram stain for bacterial detection
Budin, Ghyslain,Chung, Hyun Jung,Lee, Hakho,Weissleder, Ralph
, p. 7752 - 7755 (2012/08/29)
Magnetizing: Bacteria are often classified into Gram-positive and Gram-negative strains by staining with crystal violet (CV). The described bioorthogonal modification of CV with trans-cyclooctene (TCO) can be used to render Gram-positive bacteria magnetic
Phenylazo-Substituted Triphenylmethylium Systems
Hellwinkel, Dieter,Fritsch, Helmut
, p. 2207 - 2226 (2007/10/02)
Triphenylmethanols (or their ethers) bearing phenylazo groups react with trifluoroacetic acid to give deeply colored solutions of the tritylium ions 7(+) - 9(+), (RC6H4N=NC6H4)nC(+)(C6H5)3-n (R = H, Me, Cl, NO2, MeO, H2N, Me2N), whose long wavelengths VIS absorptions and lowfield 1H-NMR signals are consistent with the existence of through-conjugated ?-electron systems.After addition of trifluoromethanesulfonic acid significant hypsochromic shifts of the VIS bands as well as additional proton deshieldings in the 1H-NMR spectrum are observed, which are in accordance with the formation of proximally azo-protonated phenylazotritylium ions 7(+)*H(+), 8(+)*2H(+), and 9(+)*3H(+), (RC6H4N=N(+)H-C6H4)nC(+)(C6H5)3-n.Fully analogous acid-strength-dependent ionisation/protonation reactions result for the overall p-substituted ions 17(+), 18(+), (RC6H4N=NC6H4)C(+)(C6H4R')2 (R,R' = Me, MeO, Me2N), as well as for the azobistritylium system 10(2+), (C6H5)2C(+)C6H4N=NC6H4C(+)(C6H5)2, and its azoxy derivative 11(2+).The linearly extended bis- and trisazo systems 19(+), 22(+), MeC6H4N=N(C6H4N=N)1,2C6H4C(+)(C6H5)2 and the (phenylazo)styryl system 20(+), MeC6H4N=NC6H4CH=CHC6H4C(+)(C6H5)2 in trifluoroacetic acid do not show any significant color changes relativ to the corresponding monofunctional parent types.In trifluoromethanesulfonic acid, however, compounds 19(+), 22(+) exhibit the usual bathochromic shifts of the longest wavelenght VIS absorption accompanying the increasing protonation of the polyazo system.In the case of the tritylium ions containing azomethine bridges, 24(+), only for the methoxy derivative 24b(+) indications for the existence of the conjugatively extended tritylium system (MeOC6H4N=CHC6H4)C(+)(C6H5)2 are found.The amide derivatives 26(+)-29(+), on the other hand, presents no clues for the existence of through-conjugated systems of the kind ArC(OH)=NC6H4C(+)Ar2 and ArN=C(OH)C6H4C(+)Ar2, respectively.
