6358-51-6Relevant academic research and scientific papers
Diol appended quenchers for fluorescein boronic acid
Elfeky, Souad A.,Flower, Stephen E.,Masumoto, Naoko,D'hooge, Francois,Labarthe, Ludivine,Chen, Wenbo,Len, Christophe,James, Tony D.,Fossey, John S.
experimental part, p. 581 - 588 (2010/08/19)
Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Forster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.
Preparation of FRET reporters to support chemical probe development
Foley, Timothy L.,Yasgar, Adam,Garcia, Christopher J.,Jadhav, Ajit,Simeonov, Anton,Burkart, Michael D.
experimental part, p. 4601 - 4606 (2010/11/18)
In high throughput screening (HTS) campaigns, the quality and cost of commercial reagents suitable for pilot studies often create obstacles upon scale-up to a full screen. We faced such challenges in our efforts to implement an HTS for inhibitors of the phosphopantetheinyl transferase Sfp using an assay that had been validated using commercially available reagents. Here we demonstrate a facile route to the synthetic preparation of reactive tetraethylrhodamine and quencher probes, and their application to economically produce fluorescent and quencher-modified substrates. These probes were prepared on a scale that would allow a full, quantitative HTS of more than 350,000 compounds.
ORGANIC SOLVENT SOLUBLE METAL COMPLEX AZO DYES
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Page/Page column 8, (2010/02/11)
The present invention relates to organic solvent soluble dyes. In particular, the invention relates to an organic solvent soluble metal complex azo dyes containing chelated metal atoms.
