64646-75-9Relevant academic research and scientific papers
Photostable fluorescent probes for 3D imaging and monitoring the metabolism of lipid droplets
He, Liang,Li, Yi,Pan, Ying-Ming,Peng, Xiang-Jun,Tang, Hai-Tao,Wang, Kang-Nan
, (2020)
In addition to being associated with energy supply, lipid droplets (LDs) play vital roles in many other important physiological and some pathological processes. Fluorescent probes are useful tools for studying the biological functions of LDs. In this study, we develop three new highly fluorescent LD-specific bioprobes, which can be excited by one- and two-photon, with favorable biocompatibility. They have little crosstalk with some commonly used commercially-available green- and red-emissive probes, such as BODIPY 493/503, MitoTracker Red CMXRos (MTR) and LysoTracker Red DND-99 (LTR), thus allowing the simultaneous multicolor imaging. Notably, one of the probes, BIP-1, has superior photostability and allows excellent 3D imaging of cellular LDs. BIP-1 can be used to monitor the number and morphological changes of LDs, and the interactions of LDs with other organelles (e.g. mitochondria) under specific physiological conditions (e.g. starvation). Overall, BIP-1 is a promising alternative fluorescent bioprobe for live-cell LD-specific imaging and long-term tracking during LD-related research.
Transition-metal-free C-N and C-C formation: Synthesis of benzo[4,5]imidazo[1,2-: A] pyridines and 2-pyridones from ynones
Teng, Qing-Hu,Peng, Xiang-Jun,Mo, Zu-Yu,Xu, Yan-Li,Tang, Hai-Tao,Wang, Heng-Shan,Sun, Hong-Bin,Pan, Ying-Ming
supporting information, p. 2007 - 2012 (2018/05/23)
Herein, a transition metal-free and efficient cascade reaction of ynones with 2-methylbenzimidazoles under mild conditions has been revealed. This cascade reaction involved a Michael addition/intramolecular cycloaddition/dehydration and provided the desired benzo[4,5]imidazo[1,2-a]pyridines in moderate to good yield. Furthermore, three benzo[4,5]imidazo[1,2-a]pyridines (3d, 3i, and 3q) exhibited good activities against Hep-G2 (human liver cancer), T-24 (human bladder cancer cell), and SK-OV-3 (human ovarian cancer) cell lines with IC50 values in the range of 8.05-10.67 μmol L-1. To investigate the mechanism of the effective inhibition of cell growth, further studies, including cell cycle analysis, apoptosis ratio detection, measurement of Ca2+ generation, ROS, mitochondrial membrane potential assay, and caspase-3/9 activation, were carried out on compound 3i.
Unusual fischer reaction in the 1-aminobenzimidazole series. Synthesis of pyrido [1,2-a]benzimidazole derivatives
Kuz'menko,Komissarov,Simonov
, p. 1090 - 1095 (2007/10/02)
The reaction of N-aminobenzimidazoles with ketones under the conditions of the Fischer reaction, as a result of which derivatives of 1,3-disubstituted pyrido-[1,2-a]benzimidazoles are unexpectedly formed, was investigated. The structure of the latter was
