67081-75-8Relevant academic research and scientific papers
Efficient biocatalytic synthesis of (R)-pantolactone
Pscheidt, Beate,Liu, Zhibin,Gaisberger, Richard,Avi, Manuela,Skranc, Wolfgang,Gruber, Karl,Griengl, Herfried,Gliedera, Anton
, p. 1943 - 1948 (2008)
Screening for stereoselective cyanohydrin synthesis in 96-well plates was employed in the development of an efficient, pH-stable hydroxynitrile lyase for the conversion of sterically hindered aliphatic aldehydes. Site-saturation mutagenesis (SSM) resulted in a powerful catalyst for the stereoselective conversion of hydroxypivalaldehyde and pivalaldehyde to their corresponding (R)-cyanohydrins (ee >97%) which are used as chiral building blocks (e.g., for pantothenic acid production). Furthermore, redesigning the PaHNL5 gene and improving its expression by Pichia pastoris with the help of a new P AOX1 promoter variant and the helper protein PDI (protein disulfide isomerase) led to elevated amounts of today's most efficient biocatalyst for vitamin B5 synthesis.
