67221-67-4

Upstream product

• 67221-73-2 N-benzoyl-5'-O-(bis(4-methoxyphenyl)phenylmethyl)-P-(4-chlorophenyl)-2'-deoxycytidylyl-(3'->5')-3'-thymidylic acid, 4-chlorophenyl 2-cyanoethyl ester 
• 87324-60-5 C₆₁H₅₆Cl₂N₆O₁₆P₂ 
• 69507-62-6 (p-chlorophenyl) (β-cyanoethyl) phosphorochloridate 
• 67221-57-2 5'-O-(dimethoxytrityl)thymidine 3'-(p-chlorphenylcyanoethyl phosphate) 
• 57777-87-4 N⁴-benzoyl-5'-O-(p,p'-dimethoxytrityl)deoxycytidine 3'-<(p-chlorophenyl)(β-cyanoethyl)phosphate> 
• 62420-37-5 (p-chlorophenyl) di(1,2,4-triazolido)phosphate 
• 40615-39-2 5'-O-(4-4'-dimethoxytrityl)thymidine 
• 67219-55-0 4-N-Benzoyl-2'-deoxy-5'-O-(4,4'-dimethoxytrityl)cytidine 
• 57777-78-3 thymidine-3'-p-chlorophenyl-β-cyanoethyl-phosphate 
• 81312-43-8 5'-O-monomethoxytrityl-N-benzoyldeoxycytidine 3'-p-chlorophenyl phosphate 

Relevant academic research and scientific papers

Synthesis of Deoxyoligonucleotide Linker Fragments for Genetic Engineering Using Improved Preparative and Analytical Techniques

For studies on the expression of recombinant DNA, linker fragments have been prepared which serve to introduce specific points of cleavage on the DNA as well as the protein level.Such linkers were designed for cyanogen bromide as well as for collagenase cleavage of fused proteins.The preparation was done according to the triester synthesis scheme using improved and simpler routes to functionalized dinucleotide building blocks.Field desorption mass spectrometry was used as a routine tool for the identification and control of the purity of these units.

SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES CONTAINING A 3'-TERMINAL PHOSPHATE GROUP

Two methods for synthesizing oligodeoxyribonucleotides containing 3'-terminal phosphate groups have been developed.The first consists in the synthesis and the introduction into the oligonucleotides of a block containing a 3'-terminal bis(2-cyanoethyl) phosphate group and the second in the use of the transesterification reaction of oligonucleotides with 2-cyanoethanol in the presence of cesium fluoride.Both methods permit the fairly effective synthesis of oligodeoxyribonucleotide 3'-phosphates.