67473-53-4Relevant academic research and scientific papers
Synthesis of Deoxyoligonucleotide Linker Fragments for Genetic Engineering Using Improved Preparative and Analytical Techniques
Seliger, Hartmut,Bach, Trung-Chinh,Siewert, Gerhard,Boidol, Werner,Toepert, Michael,et al.
, p. 835 - 853 (2007/10/02)
For studies on the expression of recombinant DNA, linker fragments have been prepared which serve to introduce specific points of cleavage on the DNA as well as the protein level.Such linkers were designed for cyanogen bromide as well as for collagenase cleavage of fused proteins.The preparation was done according to the triester synthesis scheme using improved and simpler routes to functionalized dinucleotide building blocks.Field desorption mass spectrometry was used as a routine tool for the identification and control of the purity of these units.
Chemical Synthesis of the Pentadecadeoxyribonucleotide d(TTCTTCTACACACCC) by Improved Triester Approach
Rosenthal, A.,Cech, D.,Veiko, V. P.,Shabarova, Z. A.,Orezkaja, T. S.
, p. 764 - 773 (2007/10/02)
The chemical synthesis of the pentadecanucleotide d(TTCTTCTACACACCC) by improved triester approach in solution with TPS/1-Methylimidazole as condensation agent is reported.Almost all condensation were complete within 5-10 minutes giving yields over 80 percent.All intermediate triester blocks showed purity degrees of higher than 90 percent after purification by simple silica gel chromatography.After deblocking the pentadecanucleotide was purified by DEAE-cellulose 52 chromatography and desalted, then showing 100 percent purity as seen by 20 percent polyacrylamide gel electrophoresis of the 5'-32P labeled oligonucleotide.The sequence of the synthetic oligomer was determined and confirmed by using the two-dimensional mobility shift method.The pentadecanucleotide was used as a hybridisation probe for cloned human insulin c-DNA.
