6984-55-0Relevant academic research and scientific papers
Nucleoside phosphate, synthetic method and pharmaceutical application in resisting hepatitis virus of nucleoside phosphate
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, (2021/08/21)
The invention belongs to the technical field of medicinal chemistry, and particularly relates to nucleoside phosphate, a synthesis method and pharmaceutical application in anti-hepatitis virus of the nucleoside phosphate. The nucleoside phosphate provided by the invention is 2-thio-N-hydroxycytosine ribonucleoside phosphate, and the synthetic method has the advantages of simple route steps and high product yield. Experiments prove that the compound 2-thio-N-hydroxycytosine ribonucleoside phosphate has a good inhibition effect on HBV DNA secreted by HepG2.2.15 cells, has anti-hepatitis virus activity, and provides a good choice for treatment of viral hepatitis.
Alternative nucleic acid molecules and uses thereof
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, (2015/11/09)
The present disclosure provides alternative nucleosides, nucleotides, and nucleic acids, and methods of using them.
ALTERNATIVE NUCLEIC ACID MOLECULES AND USES THEREOF
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, (2016/06/15)
The present disclosure provides alternative nucleosides, nucleotides, and nucleic acids, and methods of using them.
ALTERNATIVE NUCLEIC ACID MOLECULES AND USES THEREOF
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, (2016/06/28)
The present disclosure provides alternative nucleosides, nucleotides, and nucleic acids, and methods of using them.
MODIFIED NUCLEIC ACID MOLECULES AND USES THEREOF
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, (2014/07/07)
The present disclosure provides modified nucleosides, nucleotides, and nucleic acids, and methods of using them.
Synthesis and characterization of the native anticodon domain of E. coli tRNA(Lys): Simultaneous incorporation of modified nucleosides mnm5s2U, t6A, and pseudouridine using phosphoramidite chemistry
Sundaram, Mallikarjun,Crain, Pamela F.,Davis, Darrell R.
, p. 5609 - 5614 (2007/10/03)
The anticodon domain of E. coli tRNA(Lys) contains the hypermodified nucleosides mnm5s2U and t6A at positions 34 and 37, respectively, along with a more common ψ at position 39. The combination of these three nucleotides represents one of the most extensively modified RNA domains in nature. 2-Cyanoethyl diisopropylphosphoramidites of the hypermodified nucleosides mnm5s2U and t6A were each synthesized with protecting groups suitable for automated RNA oligonucleotide synthesis. The 17 nucleotide anticodon stem-loop of E. coli tRNA(Lys) was then assembled from these synthons using phosphoramidite coupling chemistry. Coupling efficiencies for the two hypermodified nucleosides and for pseudouridine phosphoramidite were all greater than 98%. A mild deprotection scheme was developed to accommodate the highly functionalized RNA. High coupling yields, mild deprotection, and efficient HPLC purification allowed us to obtain 1.8 mg of purified RNA from a 1 μmol scale RNA synthesis. Our efficient synthetic protocol will allow for biophysical investigation of this rather unique tRNA species wherein nucleoside modification has been shown to play a role in codon-anticodon recognition, tRNA aminoacyl synthetase recognition, and programmed ribosomal frameshifting. The human analogue, tRNA(Lys,3), is the specific tRNA primer for HIV-1 reverse transcriptase and has a similar modification pattern.
Process for preparing 2-thiouracil nucleosides
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, (2008/06/13)
Some new 1-(2- or 3-deoxy-β-D-pentofuranosyl)-2-thiouracils and 1-β-D-pentofuranosyl-2-thio-6-azauracils have been prepared. Further, it has been found that the known nucleoside compound, 1-β-D-ribofuranosyl-2-thiouracil is active against Herpes virus and against L-1210 leukemia in mice. An improved method of preparing corresponding 2-0-methyluracil and 2-0-methyl-6-azauracil intermediates is described. The method preserves the β-configuration of the starting nucleoside.
