71100-59-9

Upstream product

• 62420-37-5 (p-chlorophenyl) di(1,2,4-triazolido)phosphate 
• 64325-78-6 N₆-benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyadenosine 
• 57777-86-3 5'-O-(dimethoxytrityl)-N-benzoyladenine 3'-(p-chlorophenylcyanoethyl phosphate) 

Downstream Products

• 67219-56-1 C₅₄H₅₁ClN₇O₁₃P 
• 81087-24-3 Benzoic acid (2R,3S,5R)-2-[[(2R,3S,5R)-5-(6-benzoylamino-purin-9-yl)-2-hydroxymethyl-tetrahydro-furan-3-yloxy]-(4-chloro-phenoxy)-phosphoryloxymethyl]-5-(2-isobutyrylamino-6-oxo-1,6-dihydro-purin-9-yl)-tetrahydro-furan-3-yl ester 
• 67221-59-4 N-benzoyl-5'-O-(bis(4-methoxyphenyl)phenylmethyl)-P-(4-chlorophenyl)-2'-deoxyadenylyl-(3'->5')-3'-thymidylic acid, 4-chlorophenyl 2-cyanoethyl ester 
• 162757-32-6 5'-O-4,4'-dimethoxytrityl-6-N-benzoyl-2'-deoxyadenylyl-(3'-6')-<1-(2',3'-dideoxy-4'-O-acetyl-β-D-erythro-hex-2'-enopyranosyl)thymine>p-chlorophenyl phosphate 
• 81087-26-5 C₆₇H₆₂Cl₂N₈O₁₆P₂ 
• 93778-55-3 C₆₅H₆₀ClN₁₀O₁₄P 
• 71459-59-1 N-benzoyl-5'-O-(bis(4-methoxyphenyl)phenylmethyl)-P-(4-chlorophenyl)-2'-deoxyadenylyl-(3'->5')-N-benzoyl-2'-deoxy-3'-adenylic acid, 4-chlorophenyl 2-cyanoethyl ester 

Relevant academic research and scientific papers

The evaluation of 2'3'-dideoxy-β-D-erythro-hex-2'-enopyranosyl nucleosides as potential antisense constructs: Synthesis, biophysical properties and enzymatic stability of 2'-deoxyadenosine-(3'-6')-[1-(2',3'-dideoxy-β-D-erythro-hex-2 '-enopyr anosyl)thymine] phosphate

1-(2',3'-dideoxy-β-D-erythro-hex-2'-enopyranosyl)thymine was used as a nucleoside substitute in the synthesis of the dimer ApT. CD studies on the dimer show that it adopts stacked, B-form mini-helices in solution. The title compound, relative to natural ApT, possesses an increased resistance to degradation by nucleases.

Synthesis of Deoxyoligonucleotide Linker Fragments for Genetic Engineering Using Improved Preparative and Analytical Techniques

For studies on the expression of recombinant DNA, linker fragments have been prepared which serve to introduce specific points of cleavage on the DNA as well as the protein level.Such linkers were designed for cyanogen bromide as well as for collagenase cleavage of fused proteins.The preparation was done according to the triester synthesis scheme using improved and simpler routes to functionalized dinucleotide building blocks.Field desorption mass spectrometry was used as a routine tool for the identification and control of the purity of these units.

PHOSPHOAMMONIUM, REACTIF DE PHOSPHORYLATION EN SYNTHESE OLIGONUCLEOTIDIQUE

The methylarylhalogenophosphate-pyridine-1,5-dimethyltetrazole system permits the introduction of the arylphosphodiester group either in the 3' or in 5' position of a deoxynucleoside or of a nucleotide carrying sensitive protecting groups such as the dimethoxytrityl and β-cyanoethyl groups.

Modified phosphotriester method for chemical synthesis of ribooligonucleotides. Part I. Synthesis of riboundecaadenylate and two fragments constituting the sequence of R-17 translation control signal

A modified phosphotriester method has been succesfully applied for the chemical synthesis of ribooligonucleotides.The starting material is a fully protected ribomononucleoside containing a 3'-phosphotriester group 5.The coupling reaction is performed usin