72-57-1 Usage
Chemical Properties
Different sources of media describe the Chemical Properties of 72-57-1 differently. You can refer to the following data:
1. blueish grey powder
2. Trypan Blue is a dark Blue crystalline solid or
powder.
Uses
Different sources of media describe the Uses of 72-57-1 differently. You can refer to the following data:
1. Biological stain.
2. Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples. Dyes and metabolites.
3. Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.
Definition
ChEBI: An organosulfonate salt that is the tetrasodium salt of 3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonic acid).
Preparation
3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-2,7-disulfonic acid(2 Moore) coupled.
General Description
Bluish-gray to dark blue powder.
Reactivity Profile
Direct Blue 14 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 14 is incompatible with strong oxidizing agents and strong acids.
Health Hazard
ACUTE/CHRONIC HAZARDS: Direct Blue 14 may cause irritation and may be absorbed through the skin. It is a positive animal carcinogen. When heated to decomposition Direct Blue 14 emits very toxic fumes of nitrogen oxides, sulfur oxides, carbon monoxide and carbon dioxide.
Fire Hazard
Flash point data for Direct Blue 14 are not available; however, Direct Blue 14 is probably combustible.
Biochem/physiol Actions
Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.
Safety Profile
Suspected carcinogen
with experimental carcinogenic,
neoplastigenic, and tumorigenic data. Poison
by intraperitoneal, intravenous, and
subcutaneous routes. Experimental
teratogenic and reproductive effects.
Mutation data reported. When heated to
decomposition it emits very toxic fumes of
NOx NazO, and SOx
Potential Exposure
Used in dyeing textiles; leather and
paper; as a biological stain.
Properties and Applications
blue. Blue gray powder. Soluble in water for blue, slightly soluble in soluble fiber element, insoluble in other organic solvents. The strong sulfuric acid in dark green light for blue, diluted into a red light blue; In the light of nitric acid for palm gray solution. The dye solution to join a red sulfuric acid 10% change; Add thick sodium hydroxide solution for red light purple.
Standard
Acid Resistance
Alkali Resistance
Light Fastness
Soaping
Water
Fading
Stain
Fading
Stain
ISO
4-5
4
1-2
2
2
AATCC
5
2
1-2
2
1
Standard
Acid Resistance
Fading
Stain
ISO
4-5
AATCC
5
Incompatibilities
Dust may form explosive mixture with
air. Trypan Blue is incompatible with strong oxidizing
agents. Incompatible with oxidizers (chlorates, nitrates,
peroxides, permanganates, perchlorates, chlorine, bromine,
fluorine, etc.); contact may cause fires or explosions. Keep
away from alkaline materials, strong bases, strong acids,
oxoacids, epoxides. Azo compounds can detonate. This
applies in particular to organic azides that have been sensitized
by the addition of metal salts or strong acids. Toxic
gases are formed by mixing materials of this class with
acids, aldehydes, amides, carbamates, cyanides, inorganic
fluorides, halogenated organics, isocyanates, ketones,
metals, nitrides, peroxides, phenols, epoxides, acylhalides,
and strong oxidizing or reducing agents. Flammable gases
are formed by mixing materials in this group with alkali
metals. Explosive combinations can occur with strong oxidizing
agents, metal salts, peroxides, and sulfides. Trypan
Blue is sensitive to prolonged exposure to heat.
Check Digit Verification of cas no
The CAS Registry Mumber 72-57-1 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 7 and 2 respectively; the second part has 2 digits, 5 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 72-57:
(4*7)+(3*2)+(2*5)+(1*7)=51
51 % 10 = 1
So 72-57-1 is a valid CAS Registry Number.
InChI:InChI=1/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,37-38H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-31+,40-32+;;;;
