74405-46-2

Usage

Uses

Used in Molecular Biology and Biotechnology:
IBU-DMT-DEOXYGUANOSINE SUCCINIC ACID is used as a synthetic building block for the preparation of DNA fragments, particularly in the creation of small-molecule/DNA hybrids via amide coupling. This application is essential for the development of innovative therapeutic approaches and the advancement of molecular biology research.
Used in Oligonucleotide Synthesis:
IBU-DMT-DEOXYGUANOSINE SUCCINIC ACID serves as a solid phase support for oligonucleotide synthesis, enabling the efficient and reliable production of specific DNA sequences. This application is vital for the development of targeted gene therapies, diagnostic tools, and other biotechnological applications that require precise control over DNA sequence synthesis.

InChI:InChI=1/C39H41N5O10/c1-23(2)36(48)42-38-41-35-34(37(49)43-38)40-22-44(35)31-20-29(54-33(47)19-18-32(45)46)30(53-31)21-52-39(24-8-6-5-7-9-24,25-10-14-27(50-3)15-11-25)26-12-16-28(51-4)17-13-26/h5-17,22-23,29-31H,18-21H2,1-4H3,(H,45,46)(H2,41,42,43,48,49)

Upstream product

• 108-30-5 succinic acid anhydride 
• 68892-41-1 5'-O-dimethoxytrityl-N-isobutyryldeoxyguanosine 

Relevant academic research and scientific papers

Stereospecific grignard activated coupling of a deoxynucleoside: Methylphosphonate on a polyethylene glycol support

Stereospecific synthesis of Rp and Sp methylphosphonate diastereoisomers of d(ApG) was carried out on a polymeric support, polyethylene glycol (PEG), by the Grignard reagent solution route described by Stec. The coupling yield on PEG was higher than that observed in solution, and precipitation of the PEG at each step allowed easy separation of oligomer product from soluble reactants at each step.

Solid Phase Synthesis of Oligodeoxyribonucleotides Utilizing the Phenylthio Group as a Phosphate Protecting Group

Oligodeoxyribonucleotide synthesis utilizing the phenylthio group as a phosphate protecting group was applied to the solid phase method.The base residues of deoxyguanosine and deoxyadenosine were protected with bis(isobutyryloxy)ethylene (Bibe) and phthaloyl groups to avoid the base modfication and depurination, respectively.A key synthetic intermediate of N2-isobutyryl-N1,N2-bis(isobutyryloxy)ethylenedeoxyguanosine was prepared in high yield by four-step reaction from deoxyguanosine and used for preparation of the building blocks of deoxyguanosine required for the polymer support synthesis.Two kinds of polymer supports, i.e., 1 percent cross-linked polystyrene and controlled pore glass were chosen.The latter was employed for the synthesis of dodecadeoxyribonucleotides by using an automated DNA synthesizer.