74848-84-3 Usage
Uses
Used in Molecular Biology and Genetics:
THYMIDINE, METHYL-D3 is used as a tracer for [application type] in molecular biology and genetics research for [application reason] studying DNA synthesis, replication, and repair mechanisms. Its labeled nature allows for the tracking of DNA incorporation and detection in various experimental setups.
Used in Cancer Research:
In cancer research, THYMIDINE, METHYL-D3 is used as a diagnostic tool for [application reason] identifying and monitoring cancer cells, as well as understanding the cell proliferation rates in tumors. The incorporation of the labeled thymidine into the DNA of rapidly dividing cells can provide valuable insights into the progression of the disease and the effectiveness of potential treatments.
Used in Pharmaceutical Industry:
THYMIDINE, METHYL-D3 is used as a research compound for [application reason] developing new drugs and therapies targeting DNA-related processes. Its unique properties make it a valuable tool in the design and testing of novel antiviral, anticancer, and antiparasitic agents.
Used in Radioimmunoassays:
In the field of radioimmunoassays, THYMIDINE, METHYL-D3 is used as a labeled antigen for [application reason] detecting and quantifying specific antibodies or other biomolecules. The labeled thymidine can be easily detected and measured, allowing for accurate and sensitive analysis of biological samples.
Used in DNA Sequencing:
THYMIDINE, METHYL-D3 is used as a labeled nucleotide for [application reason] DNA sequencing techniques, such as Sanger sequencing. The labeled thymidine can be incorporated into the growing DNA strand during the sequencing process, allowing for the detection and identification of specific DNA sequences.
Used in Biotechnology:
In the biotechnology industry, THYMIDINE, METHYL-D3 is used as a component for [application reason] creating labeled DNA probes, which are essential for various molecular diagnostics, gene expression analysis, and genotyping applications. The labeled thymidine enhances the sensitivity and specificity of these probes, making them more effective in their intended applications.
Check Digit Verification of cas no
The CAS Registry Mumber 74848-84-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 7,4,8,4 and 8 respectively; the second part has 2 digits, 8 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 74848-84:
(7*7)+(6*4)+(5*8)+(4*4)+(3*8)+(2*8)+(1*4)=173
173 % 10 = 3
So 74848-84-3 is a valid CAS Registry Number.
InChI:InChI=1/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7-,8-/m1/s1/i1D3
74848-84-3Relevant academic research and scientific papers
Deamination, oxidation, and C-C bond cleavage reactivity of 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxycytosine
Schiesser, Stefan,Pfaffeneder, Toni,Sadeghian, Keyarash,Hackner, Benjamin,Steigenberger, Barbara,Schroeder, Arne S.,Steinbacher, Jessica,Kashiwazaki, Gengo,Hoefner, Georg,Wanner, Klaus T.,Ochsenfeld, Christian,Carell, Thomas
supporting information, p. 14593 - 14599 (2013/10/22)
Three new cytosine derived DNA modifications, 5-hydroxymethyl-2′- deoxycytidine (hmdC), 5-formyl-2′-deoxycytidine (fdC) and 5-carboxy-2′-deoxycytidine (cadC) were recently discovered in mammalian DNA, particularly in stem cell DNA. Their function is currently not clear, but it is assumed that in stem cells they might be intermediates of an active demethylation process. This process may involve base excision repair, C-C bond cleaving reactions or deamination of hmdC to 5-hydroxymethyl-2′- deoxyuridine (hmdU). Here we report chemical studies that enlighten the chemical reactivity of the new cytosine nucleobases. We investigated their sensitivity toward oxidation and deamination and we studied the C-C bond cleaving reactivity of hmdC, fdC, and cadC in the absence and presence of thiols as biologically relevant (organo)catalysts. We show that hmdC is in comparison to mdC rapidly oxidized to fdC already in the presence of air. In contrast, deamination reactions were found to occur only to a minor extent. The C-C bond cleavage reactions require the presence of high concentration of thiols and are acid catalyzed. While hmdC dehydroxymethylates very slowly, fdC and especially cadC react considerably faster to dC. Thiols are active site residues in many DNA modifiying enzymes indicating that such enzymes could play a role in an alternative active DNA demethylation mechanism via deformylation of fdC or decarboxylation of cadC. Quantum-chemical calculations support the catalytic influence of a thiol on the C-C bond cleavage.
