75251-87-5

Upstream product

• 85381-24-4 5'-O-(dimethoxytrityl)-N-benzoyl-2'-deoxycytidine 3'-(o-chlorophenyl) phosphate triethylammonium salt 
• 80817-38-5 N⁴-benzoyl-2'-deoxycytidine-3'-O-(2-chlorophenyl-2-cyanoethyl)phosphate 
• 15074-54-1 2-chlorophenyl dichlorophosphate 
• 67219-55-0 4-N-Benzoyl-2'-deoxy-5'-O-(4,4'-dimethoxytrityl)cytidine 
• 72351-28-1 2-chlorophenyl phosphordi(1H-1-[1,2,4]triazolide) 
• 80817-35-2 N-4-benzoyl-5'-O-(dimethoxytrityl)-2'-deoxycitidine 3'-(o-chlorophenyl β-cyanoethyl phosphate) 
• 80817-46-5 bis(benzotriazol-1-yl)(2-chlorophenyl)phosphate 
• 76964-04-0 [1,2,4]Triazol-1-yl-phosphonic acid (2R,3S,5R)-5-(4-benzoylamino-2-oxo-2H-pyrimidin-1-yl)-2-[bis-(4-methoxy-phenyl)-phenyl-methoxymethyl]-tetrahydro-furan-3-yl ester 2-chloro-phenyl ester 

Relevant academic research and scientific papers

N,N-BISPHOSPHORODIAMIDIC CHLORIDE: A NOVEL COUPLING REAGENT IN THE SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES.

Use of a novel coupling reagent, N,N-bisphosphorodiamidic chloride (BOPDC) in the synthesis of oligodeoxyribonucleotides is described.This reagent allows the synthesis of phosphotriesters in high yields (70-80 percent) without detectable side reactions.Synthesis of a hexanucleotide, d(A-A-C-C-C-G) is presented as an example.

Improved Synthesis of Oligodeoxyribonucleotides by Solid-Phase Phosphotriester Method Utilizing O6--2'-deoxyguanosine Derivatives

The synthesis of oligodeoxyribonucleotides on a cross-linked polystyrene solid support utilizing stable mono- and dinucleotide phosphotriester building blocks is presented.The use of O6--2'deoxyguanosine derivatives yields cleaner DNA fragments by supressing side reactions.Modifications improving the phosphotriester methodology are presented.The purification methods and analysis of synthetic oligodeoxyribonucleotides are described.